تأثیر نانوفرمولاسیون بر سمیت عصاره‌ آبی برخی گیاهان علیه کنه‌ تارتن دولکه‌ای Tetranychus urticae و سفیدبالک پنبه Bemisia tabaci

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه یاسوج، یاسوج، ایران

چکیده

پژوهش حاضر، با هدف ارزیابی اثرات کشندگی عصاره‌های آبی و نانوفرمولاسیون‌های‌ گیاهان رزماریRosmarinus officinalis L. ، اسطوخودوس Lavandula angustifolia Mill.، اکالیپتوس Eucalyptus globulus Labill. و ترخون Artemisia dracunculus L. روی کنه‌ تارتن دولکه‌ای Tetranychus urticae Koch (کنه‌های بالغ نر و ماده) و زیره‌ سبز Cuminum cyminum L. و گشنیز Coriandrum sativum L. روی سفیدبالک پنبه Bemisia tabaci (Gennadius) (پوره‌ سن اول و سوم) در شرایط آزمایشگاهی با دمای 2 ± 25 درجه‌ سلسیوس، رطوبت نسبی 5 ± 65 درصد، دوره روشنایی 16 ساعت و به روش سمیت تماسی انجام پذیرفت. در میان عصاره‌های آبی، عصار‌ه‌ رزماری (3227 = LC50  پی‌پی ام) و از میان عصاره‌های فرموله شده، نانوفرمولاسیون‌ ترخون (1454 = LC50  پی‌پی ام) بیش‌ترین میزان سمیت را علیه کنه‌های بالغ داشتند. عصار‌ه‌ آبی و فرموله شده‌ زیره‌ سبز نیز به‌ترتیب با LC50 محاسبه شده 9639 و 6748 پی‌پی‌ام روی پوره‌های سن اول و 11670 و 9937 پی‌پی‌ام روی پوره‌های سن سوم، بیش‌ترین میزان سمیت را از خود نشان دادند. پوره‌های سن اول سفیدبالک نسبت به پوره‌های سن سوم، حساسیت بیش‌تری نسبت به عصاره‌های آبی و فرموله شده از خود نشان دادند. بر اساس نتایج کروماتوگرافی مایع با کارایی بالا (HPLC)، ترکیب سالیسیک اسید برای گیاهان رزماری (68/21%)، اسطوخودوس (90/33%) و گشنیز (90/9%) و کوئرستین برای اکالیپتوس (62/76%)، ترخون (49/23%) و زیره‌سبز (68/15%) بیش‌ترین درصد را داشتند. نتایج حاصل از این مطالعه نشان می‌دهد که عصاره‌های گیاهی به صورت تماسی دارای اثر کنترلی مطلوبی روی آفات گلخانه‌ای مذکور می‌باشند وکمک شایانی به استفاده از فرآورده‌های گیاهی در برنامه‌های مدیریت تلفیقی این آفات در شرایط گلخانه خواهد نمود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Nanoformulation on toxicity of some herbal extracts against two-spotted spider mite Tetranychus urticae and Silverleaf whitefly Bemisia tabaci

نویسندگان [English]

  • Farideh Arajpour
  • Roya Azizi Nesar
  • Amin Sedaratian-Jahromi
  • Mojtaba Ghane Jahromi
Department of Plant Protection, Faculty of Agriculture, Yasouj University, Yasouj, Iran
چکیده [English]

The present study was designed to evaluate the lethal effects of aqueous extracts and nanoformulations of Rosmarinus officinalis L., Lavandula angustifolia Mill., Eucalyptus globulus Labill. and Artemisia dracunculus L. on Tetranychus urticae Koch (female and male adults) and Cuminum cyminum L. and Coriandrum sativum L. on Bemisia tabaci (Gennadius) (first and third instar) under laboratory conditions at 25 ± 2 ºC, 60% RH, and 16:8 h. photoperiod using contact toxicity method. Among aqueous and formulated extracts rosemary (LC50 = 3227 ppm) and tarragon (LC50 = 1454 ppm) had the highest toxicity against adult mites, respectively. Aqueous and formulated extracts of cumin also showed the highest toxicity with LC50 of 9639 and 6748 ppm on the first instar and 11670 and 9937 ppm on the third instar of B. tabaci, respectively. The first instar of B. tabaci showed more sensitivity to aqueous and formulated extracts than third ones. Based on the results of high-performance liquid chromatography (HPLC), Salcillic acid for rosemary (21.68%), lavender (33.90%) and coriander (9.9%), and quercetin for eucalyptus (76.62%), tarragon (23.49%) and cumin (15.68%) had the highest amount of chemical compounds. Our findings show the reliable toxicity of plant extracts and will facilitate the use of plant products in integrated pest management programs under greenhouse conditions.

کلیدواژه‌ها [English]

  • Chromatography
  • Greenhouse pests
  • Integrated pest management
  • Nanoformulation
Abou-Fakhr, H., & Mcauslane, H. J. (2006). Effect of Melia azadrach L. extraxt on Bemisia argentifolii (Hemiptera:Aleyrodidae) and its biocontrol agent eretmocerus rui (Hymenoptera: Aphelinidae). Environmental Entomology, 35(3), 740- 745. DOI: https://doi.org/10.1603/0046-225X-35.3.740.
Ahmadi, Z., Saber, M., Bagheri, M., & Mahdavinia, G. R. (2017).  Achillea millefolium essential oil and chitosan nanocapsules with enhanced activity against Tetranychus urticae. Journal of Pest Science, 91(2), 837-848. DOI: https://doi.org/10.1007/s10340-017-0912-6.
Ahmed, M. Z., Barro, P. J. De, Greeff, M. J., Ren, S.X., Naveed, M., & Qiu, B. L. (2011). Genetic identity of the Bemisia tabaci species complex and association with high cotton leaf curl disease (CLCuD) incidence in Pakistan. Pest Management Science, 67, 307-317. DOI: https://doi.org/10.1002/ps.2067.
Alichi, M., Roosta, S., Bagheri, F., & Minaei, K. (2019). Effect of golder (Rydingia persica) and myrtle (Myrtus communis) extracts in controlling Bemisia tabaci on tomato under laboratory conditions. Plant Pest Research, 9(3), 29-39. (in Farsi)
Antonious, G. F., Meyer, J. E., & Snyder, J. C. (2006). Toxicity and repellency of hot pepper extracts to spider mite, Tetranychus urticae Koch. Journal of Environmental Science and Health Part B, 41(8), 1383-1391. DOI: https://doi.org/10.1080/0360123060096419.
Attia, S., Grissa, K. L., Lognay, G., Bitume, E., Hance, T., & Mailleux, A. C. (2013). A review of the major biological approaches to control the worldwide pest Tetranychus urticae (Acari: Tetranychidae) with special reference to natural pesticides. Journal of Pest Science, 86(3), 361-386. DOI: https://doi.org/10.1007/s10340-013-0503-0.
Badawy, M. E. I., Abdelgaleil, S. A. M., Mahmoud, N. F., & Marei, A. E. S. M. (2018). Preparation and characterizations of essential oil and monoterpene nanoemulsions and acaricidal activity against two-spotted spider mite (Tetranychus urticae Koch). International Journal of Acarology, 44(7), 330-340. DOI: https://doi.org/10.1080/01647954.2018.1523225.
Bettaieb, I., Bourgou, S., Wannes, W. A., Hamrouni, I., Limam, F., & Marzouk, B. (2010). Essential oils, phenolics, and antioxidant activities of different parts of cumin (Cuminum cyminum L.). Journal of Agricultural and Food Chemistry, 58(19), 10410-10418.
Calmasur, O., Aslan, I., & Sahin, F. (2006). Insecticidal and acaricidal effect of three Lamiaceae plant essential oils against Tetranychus urticae Koch and Bemisia tabaci Genn. Industrial Crops and Products, 23(2), 140-146. DOI: https://doi.org/10.1016/j.indcrop.2005.05.003.
Carmen Duda, S., Marghitas, L. A., Dezmirean, D., Duda, M., Margaoan, R., & Bobis, O. (2015). Changes in major bioactive compounds with antioxidant activity of Agastache foeniculum, Lavandula angustifolia, Melissa officinalis and Nepeta cataria: Effect of harvest time and plant species. Industrial Crops and Products, 77, 499- 507. DOI: https://doi.org/10.1016/j.indcrop.2015.09.045.
Deng, Y., Shi, D., Yin, Z., Guo, J., Jia, R., Xu, J., Song, X., Lv, C., Fan, Q., Liang, X., & Shi, F. (2012). Acaricidal activity of petroleum ether extract of neem (Azadirachta indica) oil and its four fractions separated by column chromatograohy against Sarcoptes scabiei Var. Cuniculi larvae in vitro. Experimental Parasitology, 130(4), 475-477. DOI: https://doi.org/10.1016/j.exppara.2012.02.007.
Dua, A., Garg, G., Kumar, D., & Mahajan, R. (2014). Polyphenolic composition and antimicrobial potential of methanolic coriander (Coriandrum sativum) seed extract. International Journal of Pharmaceutical Sciences and Research, 5(6), 2302-2308.
Feyzioglu, G. C., & Tornuk, F. (2016). Development of chitosan nanoparticles loaded with summer savory (Satureja hortensis L.) essential oil for antimicrobial and antioxidant delivery applications. LWT-Food Science and Technology, 70, 104-110. DOI: https://doi.org/10.1016/j.lwt.2016.02.037.
Hu, X., Wang, Y., & Peng, B. (2014). Chitosan-capped mesoporous silica nanoparticles as pH-responsive nanocarriers for controlled drug release. Chemistry-An Asian Journal, 9(1), 319-327. DOI: https://doi.org/10.1002/asia.201301105.
Jacotet-Navarro, M., Laguerre, M., Fabiano-Tixier, A. S., Tenon, M., Feuillere, N., Bily, A., & Chemat, F. (2018). What is the best ethanol-water ratio for the extraction of antioxidants from rosemary? Impact of the solvent on yield, composition and activity of the extracts. Electrophoresis, 39, 1946-1956. DOI: https://doi.org/10.1002/elps.201700397.
Khanamani, M., Fathipour, Y., Hajiqanbar, H., & Sedaratian, A. (2012). Reproductive performance and life expectancy of Tetranychus urticae (Acari: Tetranychidae) on seven eggplant cultivars. Journal of Crop Protection, 1(1), 57-66.
Khanamani, M., Fathipour, Y., Talebi, A. A., & Mehrabadi, M. (2017). Evaluation of different artificial diets for rearing the predatory mite Neoseiulus californicus (Acari: Phytoseiidae): diet-dependent life table studies. Acarologia, 57(2), 407-419. DOI: https://dx.doi.org/10.1051/acarologia/20174165.
Kheradmand, K., Beynaghi, S., Asgari, S., & Sheykhi Garjan, A. (2015). Toxicity and repellency effects of three plant essential oils against two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae). Journal of Agricultural Science and Technology, 17, 1223-1232.
Khezrilu, B. J., & Heidari, R. (2014). The evaluation of antioxidant activities and phenolic compounds in leaves and inflorescence of Artemisia dracunculus L. by HPLC. Journal of Medicinal Plants, 13(51), ‏41-50.
Laborda, R., Manzano, I., Gamon, M., Gavidia, I., Perez-Bermudes, P., & Boluda, R. (2013). Effects of Rosmarinus officinalis and Salvia officinalis essential oils on Tetranychus urticae Koch (Acari: Tetranychidae). Journal of Industrial Crops and Products, 48, 106-110. DOI: https://doi.org/10.1016/j.indcrop.2013.04.011.
Maciel, A. D. G. S., Trindade, R. C. P., Basilio Junior, I. D., Santana, A. E. G., Silva, J. P. D., Santos, L. A. T., Silva, E. S., Freitas, J. D. D., & Nascimento, T. G. D. (2019). Microencapsulation of Annona squamosal L. (Annonaceae) seed extract and lethal toxicity to Tetranychus urticae Koch (1836) (Acari: Tetranychidae). Industrial Crops and Products, 127, 251-259. DOI: https://doi.org/10.1016/j.indcrop.2018.10.084.
Makhlof, A., Tozuka, Y., & Takeuchi, H. (2011). Design and evaluation of novel pH-sensitive chitosan nanoparticles for oral insulin delivery. European Journal of Pharmaceutical Sciences, 42(5), 445-451. DOI: https://doi.org/10.1016/j.ejps.2010.12.007.
Makhlouf, L., Meudec, E., Chibane, M., Mazauric, J. P., Slimani, S., Henry, M., Cheynier, V., & Madani, K. (2010). Analysis by high-performance liquid chromatography diode array detection mass spectrometry of phenolic compounds in fruit of Eucalyptus globulus cultivated in Algeria. Agricultural and Food Chemistry, 58, 12615-12624. DOI: https://doi.org/10.1021/jf1029509.
Mena, P., Cirlini, M., Tassotti, M., Herrlinger, K. A., Dallasta, C., & Del Rio, D. (2016). Phytochemical profiling of flavonoids, phenolic acid, terpenoids and volatile fraction of a rosemary (Rosmarinus officinalis L.) extract. Molecules, 21, 1-15. DOI: https://doi.org/10.3390/molecules21111576.
Miron, T. L., Plaza, M., Bahrim, G., Ibáñez, E., & Herrero, M. (2011). Chemical composition of bioactive pressurized extracts of Romanian aromatic plants. Journal of Chromatography A, 1218(30), 4918-4927.‏ DOI: https://doi.org/10.1016/j.chroma.2010.11.055.
Mradu, G., Saumyakanti, S., Sohini, M., & Arup, M. (2012). HPLC profiles of standard phenolic compounds present in medicinal plants. International Journal of Pharmacognosy and Phytochemical Research, 4(3), 162-167.
Munin, A., & Edwards-Levy, F. (2011). Encapsulation of natural polyphenolic compounds; A review. Pharmaceutics, 3(4), 793-829. DOI: https://doi.org/10.3390/pharmaceutics3040793.
Natrajan, D., Srinivasan, S., Sundar, K., & Ravindran, A. (2015). Formulation of essential oil-loaded chitosan-alginate nanocapsules. Journal of Food and Drug Analysis, 23(3), 560-568. DOI: https://doi.org/10.1016/j.jfda.2015.01.001.
Pavela, R. (2016). Acaricidal properties of extracts of some medicinal and culinary plants against Tetranychus urticae Koch. Plant Protection Science, 52(1), 54-63. DOI: https://doi.org/10.17221/62/2015-PPS.
Pavela, R. (2007). Possibilities of botanical insecticide exploitation in plant protection. Pest Technologies, 1, 47-52.
Pimentel, D., Zuniga, R., & Morrison, D. (2005). Update of the environmental and economic costs associated with alien-invasive species in the United States. Ecological Economics, 52, 273-288. DOI: https://doi.org/10.1016/j.ecolecon.2004.10.002.
Radulescu, C., Stihi, C., Ilie, M., Lazurca, D., Gruia, R., Olaru, O. T., Bute, O., Dulama, I. D., Stirbescu, R. M., Teodorescu, S., & Florescu, M. (2017). Characterization of phenolics in Lavandula angustifolia. Analytical Letters, 40, 1-30. DOI: https://doi.org/10.1080/00032719.2016.1264409.
Rebey, I. B., Bourgou, S., Debez, I. B. S., Karoui, I. J., Sellami, I. H., Msaada, K., Limam, F., & Marzouk, B. (2011). Effects of extraction solvents and provenances on phenolic contents and antioxidant activities of cumin (Cuminum cyminum L.) seeds. Food and Bioprocess Technology, 5(7), 2827-2836. DOI: https://doi.org/10.1007/s11947-011-0625-4.
Roh, H.S., Lim, E.G., & Kim, J. (2011). Acaricidal and oviposition deterring effects of santalol identified in sandalwood oil against two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). Journal of Pest Science, 84(4), 495-501. DOI: https://doi.org/10.1007/s10340-011-0377-y.
Ruberson, J., Nemoto, H., & Hirose, Y. (1998). Pesticides and conservation of natural enemies in pest management. Conservation Biological Control, 207-220. DOI: https://doi.org/10.1016/B978-012078147-8/50057-8.
Salehi Amiri, S., Mohammadi Sharif, M., & Hadizadeh, A. (2019). Biological effects of Sambucus ebulus L., Urtica dioica L., Rubus fructicosus Boiss. and Pteridium aquilinum (L.) Kuhn extracts against two-spotted spider mite. Iranian Journal of Medicinal and Aromatic Plants, 35(4): 565-576. DOI: https://doi.org/10.22092/ijmapr.2019.124763.2464. (in Farsi)
Santos, S. A. O., Villaverde, J. J., Silva, C. M., Neto, C. P., & Silvesre, A. J. D. (2012). Supercritical fluid extraction of phenolic compounds from Eucalyptus globulus Labill bark. The Journal of Supercritical Fluids, 71, 71-79. DOI: https://doi.org/10.1016/j.supflu.2012.07.004.
Sarraf Moayeri, H. R., Pirayeshfar, F., Bolandnazar, A. R., & Faridi, B. (2014). Fumigant toxicity of cumin, spearmint and lavender essential oils against eggs and adults of two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). Plant Pest Research, 4(3), 1-13. (in Farsi)
Sedaratian-Jahromi, A., Fathipour, Y., & Moharramipour, S. (2009). Evaluation of resistance in 14 soybean genotypes to Tetranychus urticae (Acari: Tetranychidae). Journal of Pest Science, 82(2), 163-170. DOI: https://doi.org/10.1007/s10340-008-0235-8.
Shi, G. L., Zhao, L. L., Liu, S. Q., Cao, H., Clarke, S. R., & Sun, J. H. (2006). Acaricidal activities of extracts of Kochia scoparia against Tetranychus urticae, Tetranychus cinnabarinus, and Tetranychus viennensis (Acari: Tetranychidae). Journal of Economic Entomology, 99(3), 858-863. DOI: https://doi.org/10.1093/jee/99.3.858.
Shishebour, P. (2002). Whitefly (1st ed.). Shahid Chamran University Publication. (in Farsi)
Tapondjou, A. L., Adler, C., Fontem, D. A., Bouda, H., & Reichmuth, C. (2005). Bioactivities of cymol and essential oils of Cupressus sempervirens and Eucalyptus saligna against Sitophilus zeamais Motschulsky and Tribolium confusum du Val. Journal of Stored Product Research, 41, 91-102. DOI: https://doi.org/10.1016/j.jspr.2004.01.004.
Van Leeuwen, T., Vontas, J., Tsagkarakou, A., Dermauw, W., & Tirry, L. (2010). Acaricide resistance mechanisms in the two-spotted spider mite Tetranychus urticae and other important acari: A review. Insect Biochemistry and Molecular Biology, 40(8), 563-572. DOI: https://doi.org/10.1016/j.ibmb.2010.05.008.
Vasconcelos, G. N. D., Junior, M. G. C., & Barros, R. (2006). Aqueous extracts of Leucaena leucocephala and Sterculia foetida to the control of Bemisia tabaci biotypes (Hemiptera: Aleyrodidae). Ciencia Rural, 36(5), 1353-1359.
Zargar, V., Asghari, M., & Dashti, A. (2015). A review on chitin and chitosan polymers: structure, chemistry, solubility, derivatives, and applications. ChemBioEng Reviews, 2(3), 204-226. DOI: https://doi.org/10.1002/cben.201400025.