تأثیر کلرونترانیلی پرول، فلوبن دیامید و ایندوکساکارب روی شب‏پره مینوز گوجه فرنگی و بررسی اثرات جانبی کلرونترانیلی پرول روی زنبور پارازیتوئید Trichogramma brassicae

نوع مقاله : مقاله پژوهشی

نویسندگان

1 بخش حشره شناسی موسسه تحقیقات گیاهپزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

2 بخش گیاهپزشکی، مرکز تحقیقات، آموزش کشاورزی و منابع طبیعی آذربایجان غربی، سازمان تحقیقات، آموزش و ترویج کشاورزی، ارومیه، ایران

3 بخش گیاهپزشکی، مرکز تحقیقات، آموزش کشاورزی و منابع طبیعی اردبیل(مغان)، سازمان تحقیقات، آموزش و ترویج کشاورزی، مغان، ایران

4 بخش کنترل بیولوژیک موسسه تحقیقات گیاهپزشکی کشور، ایستگاه تحقیقاتی آمل، سازمان تحقیقات، آموزش و ترویج کشاورزی، آمل، ایران

چکیده

شب­پره مینوز گوجه­فرنگی یکی از آفات مهم گوجه­فرنگی در ایران است. پژوهش حاضر به­منظور معرفی و جایگزینی حشره‌کش­های جدید و افزایش دامنه حشره‌کش­های مؤثر در کنترل آفت در قالب طرح بلوک‌های کامل تصادفی با پنج تیمار در دو گلخانه­گوجه­فرنگی در استان­های اردبیل و آذربایجان غربی به اجرا در آمد. تیمارها شامل دو دز از حشره­کش کلرانترانیلی­پرول (2/0 در هزار و 15/0 در هزار)، فلوبن دیامید (3/0 در هزار)، ایندوکساکارب (3/0 در هزار) و شاهد بودند. قبل و بعد از محلول­پاشی نمونه­برداری­ از لاروهای زنده انجام شد. ده روز بعد از آخرین نمونه­برداری، از هر تکرار 20 عدد میوه به صورت تصادفی انتخاب و درصد آلودگی میوه گوجه­فرنگی به این آفت مشخص شد. همچنین، اثرات جانبی حشره­کش کلرانترانیلی­پرول روی زنبور پارازیتوئید Trichogramma brassicae در شرایط آزمایشگاهی بررسی شد. نتایج نشان داد که کلرو­نترانیلی پرول 2/ 0در هزار، چهارده روز پس از سمپاشی، در استان‌های آذربایجان غربی و اردبیل به ترتیب با 44/84 و 25/88 درصد کارایی، بیشترین کنترل را روی شب­پره مینوز گوجه فرنگی داشت. کمترین درصد آلودگی میوه در دو استان آذر بایجان غربی و اردبیل در تیمار کلرو نترانیلی پرول با دز 2/0 در هزار به ترتیب با 66/1 و 00/10 درصد به دست آمد. کلرونترانیلی پرول با دز 2/0 و 15/0 در هزار به ترتیب باعث کاهش 87/49 و 27/21 درصدی ظهور بالغین زنبور پارازیتوئید تریکو­گراما نسبت به شاهد شد. با توجه به نتایج به دست آمده، حشره­کش کلرونترانیلی­پرول با دز 15/0 در هزار برای استفاده در برنامه­های مدیریتی شب­پره مینوز گوجه­فرنگی پیشنهاد می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of chlorantraniliprole, Flubendiamide, and Indoxacarb against tomato leaf miner, and side effects of chlorantraniliprole on the parasitoid wasp, Trichogramma brassicae

نویسندگان [English]

  • F. Shafaghi 1
  • M. Fourouzan 2
  • V. Mahdavi 3
  • M. R. Attaran 4
1 Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
2 Agricultural and Natural Resources Research Center of West Azarbaijan, Urmia, Iran
3 Agricultural and Natural Resources Research Center of Ardebil, Moghan, Iran
4 plant protection lab. (Amol) Iranian Research Institute of Rice, Agricultural Research Education and Extension Organization (AREEO), Amol, Iran
چکیده [English]

Tomato leafminer is one of the most important pests of this crop in Iran. The objective of this study was to introduce and replace new insecticides and to increase the range of effective insecticides in pest control. The experiment was conducted as a randomized complete block design with five treatments in two tomato greenhouses in Ardabil and West Azarbaijan provinces. The experimental treatments included two doses of Chlorantraniliprole, (0.2 g/lit and 0.15g/lit) Flubendiamide (0.3/1000 g/lit), Indoxacarb (0.3/1000ml/lit) and Control. The live larvae were sampled before and after spraying. Ten days after the last sampling, 20 fruits were randomly selected from each replicate and the percentage of infected tomato fruit was determined. Additionally, the side effects of Chlorantraniliprole on the parasitoid wasp Trichogramma brassicae were investigated in laboratory conditions. The results showed that the treatment of Chlorantraniliprole 0.2/1000, fourteen days after spraying in West Azarbaijan province with 84.44% and in Ardabil province with 88.25% efficiency was most effective in controlling tomato leafminer. In the two provinces of West Azarbaijan and Ardabil, Chlorantraniliprole 0.2/1000 1, with 1.66 and 10.00 percent, respectively, had the lowest number of infected fruits among the treatments. Moreover, the application of Chlorantraniliprole at 0.2 /1000 and 0.15 /1000 reduced the emergence of adult Trichogramma parasitoids by 49.87 ± 4.3% and 21.27 ± 2.4%, respectively, compared with the control. According to the obtained results Chlorantraniliprole at 0.15 /1000 is recommended for use in the management programs of this pest.

کلیدواژه‌ها [English]

  • Efficiency
  • leaf miner
  • management programs
  • tomato
Adams, A.  J., Gore, A., Catchot, F., Musser, D., Cook, N., Krishnan, and Irby, T. 2016. Residual and systemic efficacy of chlorantraniliprole and flubendiamide against corn earworm (Lepidoptera: Noctuidae) in soybean. Journal of Economic Entomology 109(6): 2411–2417.
Ashtari, S., Sabahi, Q. and Talebi Jahromi, KH. 2019. Comparison of the Insecticidal Effect of Several Compounds on Biological Parameters of Trichogramma brassicae. Pesticides in Plant Protection Sciences 8(1): 47-61. (In Farsi)
Baniameri, V. and Cheraghian, A. 2011. The current status of Tuta absoluta in Iran. International Symposium on Management of Tuta absoluta (tomato leafminer), 16-18 November Agadir, Morocco.
Bassi, A., Alber R., Wiles J.A., Rison J. L., Frost N. M., Marmor F. W. and Marcon, P. C. 2007. Chlorantraniliprole: a novel anthranilic diamide insecticide. Proceedings of XVI International Plant Protection 1: 52-59.
Bloem, S. and Spaltenstein, E. 2011. New pest response guidelines tomato leafminer (Tuta absoluta). United States Department of Agriculture, Animal and Plant Health Inspection Service.
Branco, M. C. F. H., França, M. A. and Leall, J. G. T. 2001. Use of insecticides for controlling the South American tomato pinworm and the diamondback moth: a case study. Horticultura Brasileira 19(1): 60- 66.
Brugger, K. E., Cole, P. G., Newman, I. C, Parker, N., Scholz, B., Suvagia, P., Walkerf, G. and Hammondg, T. G. 2010. Selectivity of chlorantraniliprole to parasitoid wasps. Pest Management Science 66(10): 1075-1081.
Cordova, D., Benner, E. A., Sacher, M. D., Raul, J. J., Sopa, J. S. and Lahm, G. P.2006. Anthranilic diamides : A new class of insecticides with a novel mode of action, ryanodine receptor activation. Pesticide Biochemistry and Physiology 84: 196-214.
Costa, M. A., Muscardini, V. F., Gontijo, P. D. C., Carvalho, G. A., Oliveira, R. L. D. and Oliveira, H. N. D. 2014. Sub lethal and transgenerational effects of insecticides in developing Trichogramma galloi. Ecotoxicology 23(8). DOI: 10.1007/s10646-014-1282-y.
Das, S. K., Mukherjee, I. and Roy, A. 2017. Flubendiamide as new generation insecticide in plant toxicology: A policy paper. Advances in Clinical Toxicology 2: 100-122.
Desneux, N., Wajnberg, E., Wychhuys, A. G. and Burgio, G. 2010. Biological invasion of european tomato crops by Tuta absoluta: ecology, geographic expansion and prospects for biological control. Journal of Pest Science 83: 197-215.
Dinter, A., Brugger, K. E., Frost, N. M. and Woodward, M. D. 2009. Chlorantraniliprole (Rynaxypyr): A novel DuPont™ insecticide with low toxicity and low risk for honey bees (Apis mellifera) and bumble bees (Bombus terrestris) providing excellent tools for uses in integrated pest management. Hazards of pesticides to bees – 10th International Symposium of the ICP-Bee Protection Group. 8-10 October, Bucharest. pp. 84-96.
Environmental Protection and Toxic Substances Agency Pesticide Fact Sheet (EPA). 2000. Pesticide Fact Sheet: Name of Chemical: Indoxacarb. Retrieved October 30, 2000. From https://www3.epa.gov/pesticides/chem_search/reg_actions/registration/f.
European and Mediterranean Plant Protection Organization (EPPO) 2005. Datasheet Tuta absoluta datasheet. OEPP/EPPO Bulletin 35: 434-435.
Ghaderi, S., Fathipour, Y., Asgari, S. H. And Reddy, G.V. P. 2019. Economic injury level and crop loss assessment for Tuta absoluta (Lepidoptera: Gelechiidae) on different tomato cultivars. Journal of Applied Entomology: 143 (5): 1-15.
Guedes,R. N. C., Roditakis, E., Campos, M. R., Haddi, K., Bielza, P., Siqueira, H. A. A., Tsagkarakou, A., Vontas, J. and Nauen, R. 2019. Insecticide resistance in the tomato pinworm Tuta absoluta: patterns, spread, mechanisms, management and outlook. Journal of Pest Science 92: 1329-1342.
Haddi, K., Berger, M., Bielza, P., Cifuentes, D., Field, L. M., Gorman, K. and Bass, C. 2012. Identification of mutations associated with pyrethroid resistance in the voltage-gated sodium channel of the tomato leaf miner (Tuta absoluta). Insect Biochemistry and Molecular Biology 42: 506-513.
Hassan, S. A. 1992. Guideline for the evaluation of side-effects of plant protection production trichograhma cacoeciae In: Hassan, S. A. (Ed). Guidelines for testing the effects of pesticides on beneficial organisms: description of test methods. IOBC/WPRS Bulletin. pp. 18-40.
Khani, S. Hejazi, M. J. and Karimzadeh, R. 2020. Susceptibility of eggs and larvae of tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) to some insecticides. Journal of Entomological Society of Iran 39(4): 393-402. (In Farsi)
Lietti, M. M., Botto, E. and Alzogaray, R. A. 2005. Insecticide resistance in argentine populations of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). Neotropical Entomology 34: 113-119.
Nazarpour, L., Yarahmadi, L., Saber, M. and Rajabpour, A. 2016. Short and long term effects of some bio-insecticides on Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) and its coexisting generalist predators in tomato fields. Journal of Crop Protection 5 (3): 331-342.
Oliveira, H. N., Antigo, M. R., Carvalho, G. A., Glaeser, D. F. and Pereira, F. F. 2013. Seletividade de inseticidas utilizados na cana-de-açúcar a adultos de Trichogramma galloi Zucchi (Hymenoptera: Trichogrammatidae). Bioscience Journal 29(5): 1267-1274.
Parsaeyan, E., Safavi, S. A., Saber, M. and Poorjavad, N. 2018. Effects of emamectin benzoate and cypermetrin on the demography of Trichogramma brassicae. Crop Protection 110: 269-274.
Parsaeyan, E., Saber, M., Safavi, S. A., Poorjavad. N. and Biondi, A. 2020. Side effects of chlorantraniliprole, phosalone and spinosad on the egg parasitoid, Trichogramma brassicae. Ecotoxicology 29(7): 1052-1061.
Rodriguez, J. R., Pintureau, B. and Galan, M .1994. Determinants of the color of host eggs parasitized by Trichogramma fuentesi. Entomologia Experimentalis et Applicata 70: 121–128.
Sallam, A., Soliman, M. M. and Khodary, M. A. 2015. Effectiveness of certain insecticides against the tomato leaf miner Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). Advances in Applied Agricultural Science 3(2): 54-64.
Lian-Sheng, Z., Zunnu Raen, A., Asad I. A., Kaleem, T. and Mateus R. 2022. Flubendiamide Resistance and Its Mode of Inheritance in Tomato Pinworm Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). Insects , 13(11): 1023 https://doi.org/10.3390/insects13111023.
Sohrabi, F., Modarresi, M. and Hosseini, S. J. 2015. Susceptibility of different developmental stages of tomato leafminer Tuta absoluta (Meyrick) to different insecticides under laboratory conditions. Plant Protection 38(3):1-12 (In Farsi)
Shafaghi, F., Ashtari, S., Tohidi, M. T. and Modarres Najafabadi, S. S. 2022. Effectiveness of New Insecticide, Tetraniliprole (Vayego SC200) against Tomato Leaf Miner. Journal of Iranian Plant Protection Research 36(1): 67-78 (In Farsi)
Sheikhigarjan, A., Rahmani, M., Imani, S. and Jvadzade, M. 2018. Toxicity of some new generation insecticides against tomato leaf miner moth, Tuta absoluta (Meyrick) under laboratory and greenhouse conditions. Journal of Applied Research in Plant Protection 7(1): 99-108. (In Farsi)
Silva, G., Bueno, F., Favetti, F. and Neves, P. 2018. Selectivity of chlorantraniliprole and lambda-cyhalothrin to the egg parasitoid Telenomus podisi (Hymenoptera: Platygastridae). Semina: Ciências Agrárias, Londrina 39(2): 549-564.
Silva, G. A., Marcelo, C. P., Leandro, B., Andre, L. B. C., Jander, F. R. and Raul, N. C. G. 2011. Control failure likelihood and spatial dependence of insecticide resistance in the tomato pinworm, Tuta absoluta. Pest Management Science 67: 913–920.
Siqueira, H. A. A., Guedes, R. N. C. and Picanço, M. C. 2000. Insecticide resistance in populations of Tuta absoluta (Lepidoptera: Gelechiidae). Agricultural and Forest Entomology 2(2): 147-153.
Smith, I. D., Dubosis, T., Mallogo, R., Njau, E. F., Tua, S. and Srinivansa, R. 2018. host range of the invasive tomato pest Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) on solanaceous crops and weeds in Tanzania. Florida Entomologist 101(4): 573-579.
Sterk, G., Hassan, S., Bakker, F. and Biuemel, S. 1999. Results of the seventh joint testing programme carried out by the IOBC/WPRS—Working Group Pesticides and Beneficial Organisms. Biocontrol 44: 99-117.
Terzidis, A. N., Wilcockson, S. and Leifert, C. 2014. The tomato leaf miner (Tuta absoluta): Conventional pest problem, organic management solutions?. Organic Agriculture 4(1): 43-61.
Zappala, L., Biondi, A., Alma, A., AL-Jboory, I. J., Arno, J., Bayram, L. and Chailleux, A. 2013. Natural enemies of the South American moth, Tuta absoluta in Europe, North Africa and Middle East and their potential use in pest control strategies, Journal of Pest Science 86: 635-647.