نقش هیستون داستیلاز در تکثیر ویروسAutographa californica multicapsid nucleopolyhedrovirus (AcMNPV) و پاسخ ایمنی سلول‌های Spodoptera frugiperda (Sf9)

نویسندگان

دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

چکیده

ساز­وکار­های اپی­ژنتیک مانند تغییرات هیستون­ها به­خصوص استیلاسیون و داستیلاسیون، نقش مهمی در شکل­گیری روابط متقابل میزبان -بیمارگر از طریق تنظیم بیان ژن­ها برعهده دارند که توسط آنزیم­های هیستون استیل ترانسفراز و هیستون د استیلازکاتالیز می­شود. از آنجا­که یکی از روش­های کم­خطر کنترل­کننده حشرات آفت استفاده از بیمارگر­ها می­باشد؛ بنابراین، مطالعه در زمینه شناخت نقش این ساز­وکار­ها در پاسخ به عوامل بیمارگر حشرات، نقش مهمی را در کنترل آفات ایفا می­کند. بررسی­های انجام شده نشان داد که مهارکننده­های هیستون د استیلاز تکثیر ویروس­های بیمارگر پستانداران را تحت تاثیر خود قرار می­دهند. بنابراین، در این مطالعه به بررسی نقش تریکواستاتین به­عنوان مهارکننده هیستون د استیلاز در تکثیر ویروس AcMNPV و پاسخ ایمنی ضد ویروسی سلول­های Sf9 پرداخته شد. به این منظور، سلول­ها یک و سه ساعت پیش از آلودگی ویروسی یا به صورت هم‌زمان در معرض دو غلظت 1 و 25/0 میکرومولار تریکواستاتین قرار گرفته و اثر تریکواستاتین بر تکثیر ویروس و بیان برخی از ژن­های مهم ایمنی ضد ویروسی بررسی شد. نتایج نشان داد تکثیر ویروس تحت تاثیر استفاده از این ترکیب قرار نمی­گیرد. همچنین، بیان ژن­های اصلی مسیر­های ایمنی ضد ویروسی miRNA (Ago1 و Dicer1) و siRNA (Ago2 و Dicer2) پس از استفاده از غلظت­های 1 و 25/0 میکرومولار تریکو استاتین تفاوت معنی­داری با تیمارهای شاهد نداشت. باتوجه به نتایج، ساز­وکار اپی­ژنتیکی د استیلاسیون هیستون­ها تاثیری در تکثیر ویروسAcMNPV  و سیستم ایمنی ضد ویروسی در سلول­های Sf9 نداشت.

کلیدواژه‌ها


عنوان مقاله [English]

The Role of the histone deacetylase in the replication of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) and the immune response of Spodoptera frugiperda cells (Sf9)

نویسندگان [English]

  • M. Attarianfar
  • A. Mikani
  • M. Mehrabadi
Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
چکیده [English]

Epigenetic mechanisms such as histone changes, especially acetylation and deacetylation, play an important role making host-pathogen interactions by regulating gene expression, which is catalyzed by the enzymes histone acetyltransferase and histone deacetylase. Since one of the safe pest control methods is the use of pathogens, investigating the role of these mechanisms in response to insect pathogens plays a vital role in controlling pests. Studies have shown that histone deacetylase inhibitors affect the replication of mammalian pathogenic viruses. Therefore, in this study, the role of trichostatin as a histone deacetylase inhibitor in Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) virus replication and antiviral immune response in Sf9 cells was investigated. For this purpose, the cells were exposed to two concentrations of trichostatin (1 and 0.25 μM) one and three hours before or at the same time with viral infection and trichostatin effect on virus replication and some important immune system gene expression were investigated. The results showed that virus replication was not affected using this compound. Moreover, after using concentrations of 1 and 0.25 μM trichostatin, the expression level of primary genes of antiviral immune pathways (Ago2 and Dicer2) miRNA and siRNA (Ago1 and Dicer1) did not show significant difference compare with control. In conclusion, the replication of ACMNPV virus and the antiviral immune system in Sf9 cells did not affect by the epigenetic mechanism of histone deacetylation.

کلیدواژه‌ها [English]

  • Epigenetic
  • Histone deacetylase
  • Trichostatin
  • Antiviral immunity
Asgari, S. 2013. MicroRNA functions in insects. Insect Biochemistry and Molecular Biology 43(4): 388-397.
Ayres, M. D., Howard, S. C., Kuzio, J., Lopez-Ferber, M. and Possee, R. D. 1994. The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202(2): 586-605.
Baradaran, E., Moharramipour, S., Asgari, S. and Mehrabadi, M. 2019. Induction of DNA methyltransferase genes in Helicoverpa armigera following injection of pathogenic bacteria modulates expression of antimicrobial peptides and affects bacterial proliferation. Journal of Insect Physiology 118: 103939.
Bagga, S. and Bouchard, M. J. 2014. Cell cycle regulation during viral infection. Cell Cycle Control 2:.165-227.
Bianchi, F. J., Snoeijing, I., van der Werf, W., Mans, R. M., Smits, P. H. and Vlak, J. M. 2000. Biological activity of SeMNPV, AcMNPV, and three AcMNPV deletion mutants against Spodoptera exigua larvae (Lepidoptera: Noctuidae). Journal of Invertebrate Pathology 75(1): 28-35.
De Nadal, E., Zapater, M., Alepuz, P. M., Sumoy, L., Mas, G. and Posas, F. 2004. The MAPK Hog1 recruits Rpd3 histone deacetylase to activate osmoresponsive genes. Nature 427(6972): 370-374.
Dokmanovic, M. and Marks, P. A. 2005. Prospects: Histone deacetylase inhibitors. Journal of Cellular Biochemistry 96(2): 293-304.
Ehler, L.E. 2004. An evaluation of some natural enemies of Spodoptera exigua on sugarbeet in northern California. BioControl 49(2): 121-135.
Gasmi, L., Boulain, H., Gauthier, J., Hua-Van, A., Musset, K., Jakubowska, A. K., Aury, J. M., Volkoff, A. N., Huguet, E., Herrero, S. and Drezen, J. M. 2015. Recurrent domestication by Lepidoptera of genes from their parasites mediated by bracoviruses. PLoS Genetics 11(9): e1005470.
Gaetano, C., Catalano, A., Palumbo, R., Illi, B., Orlando, G., Ventoruzzo, G., Serino, F. and Capogrossi, M. C. 2000. Transcriptionally active drugs improve adenovirus vector performance in vitro and in vivo. Gene Therapy 7(19): 1624-1630.
Glatz, R., Schmidt, O. and Asgari, S. 2003. Characterization of a novel protein with homology to C-type lectins expressed by the Cotesia rubecula bracovirus in larvae of the lepidopteran host, Pieris rapae. Journal of Biological Chemistry 278(22): 19743-19750.
Gegner, J., Baudach, A., Mukherjee, K., Halitschke, R., Vogel, H. and Vilcinskas, A. 2019. Epigenetic mechanisms are involved in sex-specific trans-generational immune priming in the lepidopteran model host Manduca sexta. Frontiers in Physiology 10: 137.
Guise, A. J., Budayeva, H. G., Diner, B. A. and Cristea, I. M. 2013. Histone deacetylases in herpesvirus replication and virus-stimulated host defense Viruses 5(7): 1607-1632.
Heitmueller, M., Billion, A., Dobrindt, U., Vilcinskas, A. and Mukherjee, K. 2017. Epigenetic mechanisms regulate innate immunity against uropathogenic and commensal-like Escherichia coli in the surrogate insect model Galleria mellonella. Infection and Immunity 85(10): 00336-17.
Herbein, G. and Wendling, D. 2010. Histone deacetylases in viral infections. Clinical Epigenetics 1(1): 13-24.
Hřebačková, J., Poljaková, J., Eckschlager, T., Hraběta, J., Procházka, P., Smutný, S. and Stiborová, M. 2009. Histone deacetylase inhibitors valproate and trichostatin A are toxic to neuroblastoma cells and modulate cytochrome P450 1A1, 1B1 and 3A4 expression in these cells. Interdisciplinary Toxicology 2(3): 205.
Ikeda, M. and Kobayashi, M. 1999. Cell-cycle perturbation in Sf9 cells infected with Autographa californica nucleopolyhedrovirus. Virology 258(1): 176-188.
Karamipour, N., Fathipour, Y., Talebi, A. A., Asgari, S. and Mehrabadi, M. 2018. Small interfering RNA pathway contributes to antiviral immunity in Spodoptera frugiperda (Sf9) cells following
 
 Autographa californica multiple nucleopolyhedrovirus infection. Insect Biochemistry and Molecular Biology 101: 24-31.
Kimbrell, D.A. and Beutler, B. 2001. The evolution and genetics of innate immunity. Nature Reviews Genetics 2(4): 256-267.
Mehrabadi, M., Hussain, M. and Asgari, S. 2013. MicroRNAome of Spodoptera frugiperda cells (Sf9) and its alteration following baculovirus infection. Journal of General Virology 94(6): 1385-1397.
Negri, I. and Jablonka, E. 2016. Epigenetics as a deep intimate dialogue between host and symbionts. Frontiers in Genetics 7: 7.
Nusinzon, I. and Horvath, C. M. 2006. Positive and negative regulation of the innate antiviral response and beta interferon gene expression by deacetylation. Molecular and Cellular Biology 26(8): 3106-3113.
PazmiñoIbarra, V., MengualMartí, A., Targovnik, A. M. and Herrero, S. 2019. Improvement of baculovirus as protein expression vector and as biopesticide by CRISPR/Cas9 editing. Biotechnology and Bioengineering 116(11): 2823-2833.
Sivapragasam, A. and Syed, A. R. 2001. The genus Spodoptera with emphasis on the ecology and natural enemies of the beet armyworm, Spodoptera exigua Hubner in Malaysia. Malaysian Plant Protection Society Newsletter 12: 6-7.
Shokrzadeh, M., Ebrahimnejad, P., Omidi, M., Shadboorestan, A. and Zaalzar, Z. 2012. Cytotoxity evaluation of docetaxel nanoparticles by culturing HepG2 carcinoma cell lines. Journal of Mazandaran University of Medical Sciences 22(90): 2-10. (in Farsi)
Strand, M. R. 2008. The insect cellular immune response. Insect Science 15(1): 1-14.
Szewczyk, B., Rabalski, L., Krol, E., Sihler, W. and de Souza, M. L. 2009. Baculovirus biopesticides- safe alternative to chemical protection of plants. Journal of Biopesticides 2(2): 209-216.
Wu, J., Zhou, Z., Hu, Y. and Dong, S. 2012. Butyrate-induced GPR41 activation inhibits histone acetylation and cell growth. Journal of Genetics and Genomics 39(8): 375-384.