تاثیر باکتری Bacillus thuringiensis‌، ویروس پلی هدرهسته ای، اسپینوزاد و امامکتین روی لارو سن سوم پروانه برگخوارچغندرقند Spodoptera exigua (Lep.:Noctuidae) در شرایط آزمایشگاهی و صحرایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه ارومیه

2 گروه گیاه پزشکی، دانشگاه آزاد اسلامی واحد مهاباد

چکیده

پروانه برگخوار چغندرقند Spodoptera exigua Hübner (Lep.: Noctuidae) یکی از آفات مهم چغندرقند (Beta vulgaris L.) می­باشد، با توجه به مشکلات ناشی از مصرف سموم شیمیایی علیه این آفت، استفاده از حشره کش ­های زیست پایه در برنامه مدیریت تلفیقی آن قابل توصیه می ­باشد. لذا در این تحقیق، تاثیر باکتری Bacillus thuringiensis subsp. kurstaki، ویروس SeNPV، اسپینوزاد و امامکتین روی لاروهای سن سوم پروانه ­برگخوار­چغندرقند در شرایط آزمایشگاهی و صحرایی مورد بررسی قرارگرفت. در شرایط آزمایشگاهی، شاخص LC50 به وسیله تجزیه پروبیت حاصل از تاثیر غلظت­ های مختلف باکتری، ویروس، اسپینوزاد و امامکتین بعد از 24، 48 و 72 ساعت و همچنین ارزیابی تاثیر هر ترکیب در شرایط صحرایی با استفاده از روش هندرسون- تیلتون و آنالیز GLM-U بعد از 1، 3، 7، 14 و 21 روز محاسبه شد. نتایج تجزیه پروبیت حاصل از غلظت­ های مختلف باکتری، اسپینوزاد و امامکتین بعد از 24، 48 و 72 ساعت به ترتیب (52/3588، 25/1، 89/1)، (87/1954، 95/0، 33/1) و (25/1843، 78/0، 20/1) میلی­گرم در لیتر و برای ویروس (105×34/5، 105×­31/4 و105×­14/4) OBs/lm به­ دست ­آمد.­ در بررسی تاثیر تیمارهای باکتری، ویروس، اسپینوزاد و امامکتین در شرایط صحرایی بعد از 21 روز بیشترین و کمترین تلفات به ترتیب مربوط به تیمار امامکتین(33/61) و ویروس)00/32%( گزارش شد. در ارزیابی خسارت، بیشترین خسارت مربوط به تیمار ویروس بعد از شاهد و کمترین در تیمار امامکتین و سپس اسپینوزاد مشاهده شد. بر اساس زیست ­سنجی آزمایشگاهی و صحرایی سموم اسپینوزاد و امامکتین، موثرترین ترکیبات در کنترل این آفت و ویروس و باکتری در مدیریت تلفیقی قابل­ توصیه می ­باشند.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Bacillus thuringiensis, SeNPV, Spinosad and Emamectin on third larval instar of Spodoptera exigua (Lep.: Noctuidae) in laboratory and field conditions

نویسندگان [English]

  • M. Abdollahzadeh Bavani 1
  • Sh. Aramideh 1
  • A. Hosseinzadeh 2
1 Department of Plant Protection, Agricultural Faculty, Urmia University, Iran
2 Department of Plant Protection, Mahabad Branch, Islamic Azad University, Mahabad, Iran
چکیده [English]

The beet armyworm, Spodoptera exigua Hübner is one of the most important pests of sugar beet. Due to the problems associated with the use of chemical pesticides against of this pest, the use of bio-pesticides in integrated management program is recommended. Therefore, in this research, effects of Bacillus thuringiensis subsp. kurstaki, SeNPV virus, Spinosad and Emamectin on 3rd larval instar of beet armyworm in laboratory and field conditions were evaluated. In laboratory experiments, LC50 values of different concentrations of B. thuringiensis, SeNPV virus, Spinosad and Emamectin after 24, 48 and 72 hours were determined by probit analysis. Also, the effects of each compound using Henderson- Tilton method and GLM analysis after 1, 3, 7, 14 and 21 days were evaluated in field conditions. The results of probit analysis of different concentrations of Bt., Spinosad and Emamectin after 24, 48 and 72 hours were (3588.5, 1.25, 1.89), (1954.87, 0.95, 1.33) and (1843.25, 0.78, 1.20) mg/L, and for SeNPV (5.34×105, 4.31×105, 4.14 ×105) OBs.ml, respectively. Also, the highest and lowest mortality due to Bt., SeNPV, Spinosad and Emamectin in field conditions after 21 days were related to Emamectin (61.33) and SeNPV virus (32.00) %. The highest and lowest percentages of damage were observed in the SeNPV virus, control and the Emamectin and Spinosad, respectively. Based on laboratory and field bioassay, Spinosad and Emamectin, as the most effective compounds for controlling this pest and virus and Bt for integrated pest management are recommended.

کلیدواژه‌ها [English]

  • Beet armyworm
  • biological control
  • Bio-pesticides
  • Damage

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تحقیقات آفات گیاهی
دوره 9، شماره 1
خرداد 1398
صفحه 1-12

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  • تاریخ دریافت: 11 دی 1348
  • تاریخ پذیرش: 11 دی 1348

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