ارزیابی مقاومت ژنوتیپ‌های جو وحشی Hordeum spontaneum به شته روسی گندم Diuraphis noxia

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام

2 گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه ایلام

3 دانشکده کشاورزی دانشگاه پیام نور کرج

چکیده

 شته روسی گندم Diuraphis noxia یکی از  آفات مهم  جو است که ناقل بیماری­های ویروسی نیز می­باشد. در این تحقیق مقاومت 30 نمونه از ژنوتیپ­های جو وحشی Hordeum spontaneum، شامل نمونه­هایی از مناطق مختلف تنوع جو در جهان، نسبت به شته روسی گندم در گلخانه ارزیابی شد. در این آزمایش در مرحله دو برگی، آلوده­سازی با شته روسی گندم انجام شد و 21 روز بعد از آلودگی، ارزیابی به روش کیفی و بر اساس میزان کلروز و لوله شدن برگ،  با استفاده از مقیاس نه درجه­ای وبستر و همکاران انجام شد. همچنین محتوای کلروفیل a، b و کلروفیل کل برگ ژنوتیپ­ها اندازه­گیری شد. نتایج نشان داد تنوع ژنتیکی زیادی بین ژنوتیپ­ها از لحاظ میزان کلروز و لوله شدن برگ وجود دارد، همچنین میزان کلروز با کلروفیل a و b همبستگی مثبت و معنی­داری داشت. ژنوتیپ­های 401552IG، 140073IG، 120794IG، 40154IG، 142356IG، 119447IG، 40155IG، 38669IG،  140189IG، 1IG و 2IG از نظر تمامی صفات مورد بررسی در گروه مقاوم جای گرفتند.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of resistance to Russian Wheat aphid, Diuraphis noxia in genotypes of wild barely, Hordeum spontaneum

نویسندگان [English]

  • N. Voojudy Yekta 1
  • Z. Tahmasebi 1
  • M. Mirab-balou 2
  • F. Fatehi 3
1 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam, Iran
2 Department of Plant Protection, Faculty of Agriculture, Ilam University, Ilam, Iran
3 Department of Agriculture, Payam Noor University (PNU), Tehran, Iran.
چکیده [English]

 Russian Wheat Aphid, Diuraphis noxia is an important pest on barely, which is also the vectors of viral diseases. In order to identify resistant genotypes, resistance of 30 wild barley genotypes, including international Hordeum spontaneum germplasms, was evaluated in the greenhouse experiments. In this experiment, plants infested with aphids at two-leaf stage and 21 days after infestation, were assessed qualitatively for leaf rolling and leaf chlorosis, based on a scale from 1 to 9 In the experiment, the content of chlorophyll a, b and total chlorophyll were evaluated. Results showed genetic variation among genotypes for leaf chlorosis and leaf rolling. Moreover, a significant positive correlation was found between leaf chlorosis and chlorophyll a and b, in general, according to all traits, genotypes of IG401552, IG140073, IG120794, IG40154, IG142356, IG119447, IG40155, IG38669, IG140189, IG1 and IG2 were placed in resistant group.

کلیدواژه‌ها [English]

  • Chlorophyll
  • leaf chlorosis
  • Leaf rolling

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تحقیقات آفات گیاهی
دوره 7، شماره 3
دی 1396
صفحه 9-22

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  • تاریخ دریافت: 04 دی 1396
  • تاریخ پذیرش: 04 دی 1396

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