ارزیابی مقاومت ژنوتیپ‌های جو وحشی Hordeum spontaneum به شته روسی گندم Diuraphis noxia

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام

2 گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه ایلام

3 دانشکده کشاورزی دانشگاه پیام نور کرج

چکیده

 شته روسی گندم Diuraphis noxia یکی از  آفات مهم  جو است که ناقل بیماری­های ویروسی نیز می­باشد. در این تحقیق مقاومت 30 نمونه از ژنوتیپ­های جو وحشی Hordeum spontaneum، شامل نمونه­هایی از مناطق مختلف تنوع جو در جهان، نسبت به شته روسی گندم در گلخانه ارزیابی شد. در این آزمایش در مرحله دو برگی، آلوده­سازی با شته روسی گندم انجام شد و 21 روز بعد از آلودگی، ارزیابی به روش کیفی و بر اساس میزان کلروز و لوله شدن برگ،  با استفاده از مقیاس نه درجه­ای وبستر و همکاران انجام شد. همچنین محتوای کلروفیل a، b و کلروفیل کل برگ ژنوتیپ­ها اندازه­گیری شد. نتایج نشان داد تنوع ژنتیکی زیادی بین ژنوتیپ­ها از لحاظ میزان کلروز و لوله شدن برگ وجود دارد، همچنین میزان کلروز با کلروفیل a و b همبستگی مثبت و معنی­داری داشت. ژنوتیپ­های 401552IG، 140073IG، 120794IG، 40154IG، 142356IG، 119447IG، 40155IG، 38669IG،  140189IG، 1IG و 2IG از نظر تمامی صفات مورد بررسی در گروه مقاوم جای گرفتند.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of resistance to Russian Wheat aphid, Diuraphis noxia in genotypes of wild barely, Hordeum spontaneum

نویسندگان [English]

  • N. Voojudy Yekta 1
  • Z. Tahmasebi 1
  • M. Mirab-balou 2
  • F. Fatehi 3
1 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam, Iran
2 Department of Plant Protection, Faculty of Agriculture, Ilam University, Ilam, Iran
3 Department of Agriculture, Payam Noor University (PNU), Tehran, Iran.
چکیده [English]

 Russian Wheat Aphid, Diuraphis noxia is an important pest on barely, which is also the vectors of viral diseases. In order to identify resistant genotypes, resistance of 30 wild barley genotypes, including international Hordeum spontaneum germplasms, was evaluated in the greenhouse experiments. In this experiment, plants infested with aphids at two-leaf stage and 21 days after infestation, were assessed qualitatively for leaf rolling and leaf chlorosis, based on a scale from 1 to 9 In the experiment, the content of chlorophyll a, b and total chlorophyll were evaluated. Results showed genetic variation among genotypes for leaf chlorosis and leaf rolling. Moreover, a significant positive correlation was found between leaf chlorosis and chlorophyll a and b, in general, according to all traits, genotypes of IG401552, IG140073, IG120794, IG40154, IG142356, IG119447, IG40155, IG38669, IG140189, IG1 and IG2 were placed in resistant group.

کلیدواژه‌ها [English]

  • Chlorophyll
  • leaf chlorosis
  • Leaf rolling
Burd, J. D., Burton R. L. and Webster, J. A. 1993. Evaluation of Russian wheat aphid (Hom.: Aphididae) damage on resistant and susceptible host with comparisons of damage ratings to quantitative plant measurements. Journal of Economic Entomology 86: 974-980.

Burd, J. D. and Elliott, N. C. 1996. Changes in chlorophyll a fluorescence induction kinetics in cereals infested with Russian wheat aphid (Homoptera: Aphididae). Journal of Economic Entomology 89: 1332–1337.

Calhoun, D. S., Burnett, P. A., Robinson, J. R. and Vivar, H. E. 1991. Field resistance to Russian wheat aphid in barley: I. Symptom expression. Crop Science 31: 1464-1467.

Damsteegt, V. D., Gildow, F. E., Hewings, A. D. and Carrol, T. W. 1992. A clone of the Russian wheat aphid as a vector of the barley yellow dwarf, barley stripe mosaic and brome mosaic viruses. Plant Diseases 76(11): 1155 -1160.

Dori, H. R., Asad, M. and Emam E. 1998.Inheritance of resistance to Russian aphid by leafchlorosisinvestigation,Proceeding of 5th National Iranian Crop Science Congress. 30 August- 2 September, Iran. pp 400. (In Farsi).

Du Toit, F. 1993. Inheritance of resistance in two Triticum aestivum lines to Russian wheat aphid (Hom.: aphididae). Journal of Economic Entomology 82(4): 1251-1253. 

El-Tayeb, M. A. 2005. Response of barley grain to the interactive effect of salinity and salicylic acid. Plant Growth Regulation 42: 215-224.

Ennahli, S., El Bouhssini, M. and Grando, S. 2009. Comparison of categories in wheat and barley genotypes against biotype 2 of the Russian wheat aphid .Arthropod- Plant Interactions 3: 45 -53.

Franzen, L. D., Gutsche, A. R. and Heng-Moss, T. M. 2007. Physiological and biochemical responses of resistant and susceptible wheat to injury by the Russian wheat aphid, Diuraphis noxia (Mordvilko). Journal of Economic Entomology 100: 1692–1703.

Gray, M. E, Hein, G. L., Walgenbach, D. D. and Elliott, N. C. 1990.Effects of Russian wheat aphid (Homoptera: Aphididae) on winter and spring wheat infested during different plant growth stages under greenhouse conditions. Journal of Economic Entomology 83: 2434-2442.

Hamedanian, P., Hosseinzadeh, A., Najafi Mirak, T. and Nourbakhsh A. H. 2010. Evaluation of resistance of bread wheat cultivars to Russian wheat aphid at seedling and stem elongation growth stages. Seed and plant Improvement Journal 26(1): 27-43.

Heng-Moss, T. M., Ni, X., Macedo, T., Markwell, J. P., Baxendale, F. P., Quisenberry, S. S. and Tolmay, V. 2003. Comparison of chlorophyll and carotenoid concentrations among Russian wheat aphid (Homoptera: Aphididae)-infested wheat isolines. Journal of Economic Entomology 96: 475–481

Kazemi, M. H., Talebi-Chaichi, P., Shakiba, M. R. and Mashhadi Jafarloo, M. 2001. Comparison resistance of five wheat cultivars to the Russian wheat aphid, Diuraphis noxia (Mordvilko) (Homoptera: Aphididae) in greenhouse condition. Proceeding of 14th Iranian plant protection congress. 8 - 5 September, Iran. pp. 599.(In Farsi)

Kindler, S. D. and Springer, T. L. 1991. Resistance to Russian wheat aphid in wild Hordeum species. Crop Science 31: 94-97.

Kindler, S., Springer, T. and Jensen, K. 1995. Detection and characterization of the Rusian wheat aphid (Hom : Aphididae) in tall wheatgrass. Journal of Economic Entomology 88(5):1503 -1509.

Kindler, S., Jensen, B and Springer, L. 1993. Resistance to the Russian wheat aphid within the perennial Triticeae. Journal of Economic Entomology 86:1609 -1618.

Lichtenthaler, H. K. 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods in Enzymology 22(6): 23-24.

Matus, I. A. and Hayes, P. M. 2002. Genetic diversity in three groups of barley germplasm assessed by simple sequence repeats. Genome 45: 1095-1106.

Mayer, K. F. X., Waugh, R., Langridge, P., Close, T. J., Wise, R. P., Graner, A., Matsumoto, T. and Sato, K. 2012. A physical, genetic and functional sequence assembly of the barley genome. Nature 2(1): 10-16.

Miller, H., Porter, D. R. and Burd, J. D. 1994. Physiological effects of Russian wheat aphid (Homoptera: Aphididae) on resistant and susceptible barley. Journal of Economic Entomology 87:493–499.

Miller, R. 1992. Insect pests of wheat and barley of Mediterranean, Africa and west Asia. ICARDA. SYRIA. P. 19.

Mornhinweg, D. H., Porter, D. R. and Webster, J. A. 1995. Inheritance of Russian wheat aphid resistance in spring barley. Crop Science 35: 1368-1371.

Najafi Mirak, T., Zali, A., Hosseinzadeh, A., Rassoulian, G. R. and Saidi, A. 2004b.Evaluation of resistance to Russian wheat aphid, Diuraphis noxia (Mordvilko) in durum and bread wheats. Journal of Agriculture and Natural Resource 7(4): 115-127(in Farsi).

Najafi Mirak, T., Zali, A., Hosseinzadeh, A., Saidi, A. and Rassoulian, G. R. 2004a.Inheritance and allelism of resistance to Russian wheat aphid, Diuraphisnoxia (Mordvilko) in Iranian wheat cultivars. International Journal of Agriculture and Biology 6: 525-528.

Namavar A. 2013. Evaluation resistance of varieties of wheat to Russian wheat aphid, Diuraphis noxia(Mordvilko). Ms.C thesis. Ilam University(In Farsi).

Nematollahy M. R. 2010. Evaluation of Russian wheat aphid resistance in cultivars and advance lines of wheat under field conditions in Isfahan province, Iran. Journal of Entomological Society of Iran 30(1): 1-13.

Nematollahy M. R. and Ahmadi A. A. 1998. Characterization of resistance to Russian wheat aphid, Diuraphis noxia (Mordvilko), in several wheat (Triticum spp.) genotypes. Iran Agricultural Research 18(2): 91-106.

Nematollahy M. R., Ahmadi A. A., Emam Y. and Assad M. T. 1998. Evaluation of wheat (Triticum spp.) resistance to the Russian wheat aphid (Diuraphis noxia Mordvilko). Iran Agricultural Research 17(1): 1-18

Neocleous, D. and Vasilakakis, M. 2007. Effects of NaCl stress on red raspberry (Rubus idaeus L.). Scientia Horticulturae 112: 282-289.

Nkongolo, K. K., Quick, J. S., Limin, A. E, Fowler, D. B., PeaIirs, F. B. and Meyer, W. L. 1990. Russian wheat aphid (Diuraphis noxia) resistance in wheat and related species. Journal of Plant Science 70: 691-698.

Porter, D. R., Webster, J. A. and Baker, C. A. 1993.Detection of resistance to Russian wheat aphid in hexapleoid. Plant Breeding 110: 160-175.

Pourhaji, A. 2012. Mass screening of 76 barley genotypes for resistance to Russian wheat aphid Diuraphis noxia (Hom.: Aphididae), in greenhouse condition. Entomology and Phytopathology 79(1): 1-16(In Farsi).

Rafi, M., Zemetra, M. and Quisenberry, S. S. 1997. Feeding damage of Russian wheat aphid on resistant and susceptible wheat genotypes. Cereal Research Communications 25: 63–68.

Scott, R. A., Worrall, W. D. and Frank, W. A. 1991. Screening of resistance to Russian wheat aphid in triticale. Crop Science 31: 32-36.

Shekarian, B. 1998. Evaluation resistance of varieties of wheat to Russian wheat aphid, Diuraphis noxia (Mordvilko) in Karaj. Msc. thesis, Tehran University (In Farsi).

Shekarian, B., Rassoulian, G. R. and Azemayesh Fard, P. 2001.Screening of different varieties of wheat to find resistance sources to Russian wheat aphid, Diuraphis noxia (Mordvilko). Iranian Journal of Agricultural Sciences 32: 253-237 (in Farsi).

Shekarian, B., Rassoulian, G. R., Azemayesh Fard, P. and Ganadha, M. R. 2000.Evaluation resistance of varieties of wheat to Russian wheat aphid, Diuraphis noxia (Mordvilko). Proceeding of 14th Iranian plant protection congress. 8-5 September, Iran. pp. 599(In Farsi).

Souriai, S. and Hmitri, S. 2002. Laboratory screening barley genotype seedlings for aphid resistance in Egypt. Journal of Agricultural Research 80: 1.

Webster, J. A., Starks, K. J. and Burton, R. L. 1987. Plant resistance studies with the Diuraphis noxia (Homoptera: Aphididae), a new United States wheat pest. Journal of Economic Entomology 80: 944-949.

Webster, J. A., Porter, D. R., Baker, G. A. and Mornhinweg, D. W. 1993: Resistance to Russian wheat aphid (Homoptera: Aphididae) in barley: Effects on aphid feeding. Journal of Economic Entomology 86(5): 1603-1608.