بهینه سازی مصرف سم دلتامترین علیه شب پره موم خوار بزرگ Galleria mellonella L. با روش سطح پاسخ در شرایط آزمایشگاهی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشکده کشاورزی، دانشگاه صنعتی شاهرود، شاهرود

2 گروه گیاه پزشکی سازمان پژوهش‌های علمی و صنعتی ایران، تهران

چکیده

شب­ پره موم­ خوار بزرگ Galleria mellonella L. یکی از آفات بسیار مهم محصولات انباری و کندو­های زنبور عسل است که امروزه برای کنترل آن از سموم شیمیایی استفاده می­شود. در صورت استفاده نامناسب از سموم، مشکلات جدی زیست­محیطی و همچنین عوارض مزمنی روی سلامت انسان ایجاد می­ شود. با استفاده بهینه از این مواد، خسارت این آفت کاسته می­شود. منظور از استفاده بهینه، انتخاب الگوی صحیح و به­کارگیری روش­های مناسب در مصرف سم است. لذا در این پژوهش از روش سطح پاسخ به منظور تعیین نقاط بهینه مصرف حشره­ کش دلتامترین جهت دستیابی به بیشینه مرگ و میر با کمترین دز مصرفی استفاده شد. تاثیر عوامل دما (35-25 درجه سلسیوس)، رطوبت (80-70 %) و دز مصرف (500-200 میکرولیتر) بر میزان مرگ و میر لارو سن پنج شب پره موم خوار مورد ارزیابی قرار گرفت. آزمایش­ ها بر اساس طرح مرکب مرکزی، انجام شد. بیشینه مرگ و میر با این روش 6/7 عدد لارو سن پنج به­ ترتیب در دمای 25 درجه سلسیوس، رطوبت 70 % و دز 200 میکرولیتر در 1000 میلی­ لیتر آب تعیین شد. نتایج آزمایش بیانگر تاثیر خطی معنی ­دار دز مصرفی و دما روی مرگ و میر لارو سن پنج شب پره موم خوار بزرگ است. 

کلیدواژه‌ها


عنوان مقاله [English]

Optimization of using pesticide deltamethrin against Galleria mellonella by response surface method in laboratory conditions

نویسندگان [English]

  • A. Shabani nejad 1
  • M. Ajamhassani 1
  • B. Tafaghodinia 2
1 Departmant of Plant Protection, Faculty of Agriculture, Shahrood University of Technology
2 Departmant of Plant Production and Sustainable Agriculture, Iranian Research Organization for Science and Technology, Tehran, Iran
چکیده [English]

The Greater Wax moth, Galleria mellonella Lin. is one of the most important pests of stored products and beehives. Chemical pesticides are used for its control. Inappropriate using of pesticides caused serious environmental problems and also chronic effects on human health. Pest damage is reduced by the optimum use of these materials. The purpose of optimum use is selection of the correct pattern and applying appropriate methods for use of toxins. So in this study response surface method was used in order to determine optimal points to achieve maximum mortality with minimum dosage. The effect of factors such as temperature (25-35 °C), humidity (70-80 %) and the dosage (200-500 µL) on the mortality of fifth instar larvae of greater wax moth was evaluated. The experiments were performed according to Central Composite Design. Using the method, the maximum mortality was determined 7.6 fifth instar larvae of greater wax moth at 25 °C, humidity 70% and 200 μl of deltamethrin in 1000 ml of water. The result showed that temperature and dosage had significant effect on mortality of fifth instar larvae of Greater Wax moth.

کلیدواژه‌ها [English]

  • Greater Wax Moth
  • Deltametrin
  • Response Surface Method
  • Optimization
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