تاثیر اگزیندول و حشره‌کش بیولوژیک بایولپ® بر سمیت، شاخص‌های تغذیه‌ای، آنزیم‌های گوارشی و ذخایر انرژی کرم قوزه پنبهHelicoverpa armigera Hubner (Lep.: Noctuidae)

نوع مقاله : مقاله پژوهشی


1 گروه گیاه‌پزشکی دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

2 گروه گیاه‌پزشکی دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران


 اگزیندول (Oxindole) یک مهار کننده سیگنال ایکوزانویید است و سامانه ایمنی در حشرات را مهار می­کند. یکی از مهم‌ترین مشکلات عوامل بیولوژیک نظیر باکتریBacillus thuringiensis Kurstaki (BtK)  کارایی پایین و کندی اثرگذاری آن­ها است. در این تحقیق اثر اگزیندول و فرآورده بیولوژیک بایولپ® بر پایه BtK، روی سمیت، شاخص­های تغذیه‌ای، ذخایر انرژی و فعالیت آنزیم­های گوارشی کرم قوزه پنبه Helicoverpa armigera Hubner (Lep.: Noctuidae) مورد بررسی قرار گرفت. نتایج نشان داد اگزیندول باعث افزایش تلفات لاروها و کاهش LC50 و LT50 بایولپ® شد. شاخص‌های تغذیه­ای کرم قوزه پنبه تحت تاثیر غلظت­های زیرکشنده باکتری و اگزیندول تغییر یافت. در غلظت 5/0 گرم اگزیندول یر کیلوگرم غذا نسبت به شاهد، نرخ رشد نسبی کاهش و شاخص قابلیت هضم شوندگی افزایش نشان داد. کاربرد اگزیندول همراه با باکتری باعث کاهش فعالیت آنزیم گوارشی لیپاز نیز شد. گلیکوژن به عنوان یک منبع ذخیره انرژی در غلظت‌های 1/0 و 5/0گرم اگزیندول بر کیلوگرم غذا همراه با غلظت زیرکشنده بایولپ® نسبت به غلظت زیرکشنده بایولپ® به تنهایی، کاهش نشان داد. نتایج این تحقیق نشان داد با افزودن اگزیندول به عنوان یک بازدارنده سیگنال ایکوزانویید و سامانه ایمنی به رژیم غذایی کرم قوزه پنبه، اثرگذاری حشره­کش بایولپ® افزایش یافته و فیزیولوژی تغذیه آفت تحت تاثیر قرار گرفت.


عنوان مقاله [English]

The effect of oxindole and biological insecticide Biolep®on toxicity, feeding indices, digestive enzymes and energy reserves of American cotton bollworm, Helicoverpa armigera Hubner (Lep.: Noctuidae)

نویسندگان [English]

  • A. Baghban 1
  • G. Moravvej 1
  • J. Jalali Sendi 2
1 Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
2 Department of Plant Protection, Faculty of Agricultural Sciences, Guilan University, Rasht, Iran
چکیده [English]

Oxindole is an inhibitor of the eicosanoid signal leading to inhibiting the immune system of insects. One of the important problems of using biological agents such as Bacillus thuringiensis Kurstaki (BtK) is their low efficiency and slowness of effect. In this research, the effect of oxindole and Biolep® based on BtK, were investigated on toxicity, feeding indices, energy reserves, and digestive enzyme activity of Helicoverpa armigera Hubner (Lep.: Noctuidae) larvae. The results showed that oxindole increased larval mortality and decreased LC50 and LT50 values of Biolep®. The feeding indices of cotton bollworm were changed under the influence of sub-lethal concentrations of bacteria and oxindole. At the concentration of 0.5 g oxindole/ kg diet the relative growth rate (RGR) decreased and the approximate digestibility (AD%) increased compared to the absence of oxindole. The use of oxindole along with bacteria also decreased lipase activity. Glycogen as an energy reserve was adversely affected by simultaneous application of oxindole concentrations of 0.1 or 0.5 g.kg-1 and the sub-lethal concentrations of Biolep® compared to sub-lethal concentrations of Biolep® only. The results of this research showed that by adding oxindole as an inhibitor of eicosanoid signal and immune system to the diet of cotton bollworm, the effectiveness of Biolap® increased and the feeding physiology of the pest was affected.

کلیدواژه‌ها [English]

  • Bacillus thuringiensis
  • energy reserves
  • bioassay
  • feeding indices
  • Oxindole
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