اجزای شیمیایی، سمیت تدخینی و تاثیر بیوشیمیایی و ضد تغذیه‌ای اسانس Eucalyptus oleosa Muell. روی سوسک کشیش، Rhyzopertha dominica (F.)

نوع مقاله : مقاله پژوهشی

نویسنده

گروه تولیدات گیاهی، دانشکده کشاورزی و منابع طبیعی مغان، دانشگاه محقق اردبیلی، اردبیل، ایران.

10.22124/iprj.2022.5793

چکیده

سوسک کشیش، Rhyzopertha dominica (F.)، باعث ایجاد خسارات کمی و کیفی گسترده­ای به دانه­های غلات انباری می­شود. استفاده از ترکیبات شیمیایی ساختگی در مدیریت چنین آفاتی آثار جانبی زیادی از قبیل آلودگی محیط زیست، تهدید سلامتی انسان و توسعه مقاومت آفات را در پی داشته است. توانمندی حشره­کشی نویدبخش اسانس­های استخراج شده از گونه­های مختلف جنس Eucalyptus روی حشرات آفت در پژوهش­های اخیر نشان داده شده است. در این تحقیق، تاثیر کشندگی و زیرکشندگی اسانس E. oleosa Muell. روی حشرات کامل R. dominica بررسی شد. تجزیه شیمیایی اسانس نشان داد که ترکیبات ترپنی نظیر 1,8-cineole، α-pinene، trans-pinocarveol، (S)-α-terpineol و β-eudesmol واجد بالاترین مقدار بودند. حشرات کامل در برابر تدخین اسانس حساس بودند؛ به­طوری­که مقادیر LC50 (غلظت کشنده 50 درصد) محاسبه شده بعد از 24، 48 و 72 ساعت به­ترتیب 923/42، 828/35 و 044/34 میکرولیتر بر لیتر هوا برآورد شد. با وجود افزایش فعالیت آنزیم­های آلفا- و بتا-استراز، محتوای پروتئین، گلیکوژن و لیپید کل و فعالیت­های آمیلولیتیک و پروتئولیتیک حشرات کامل تیمار شده با LC30 (غلظت کشنده 30 درصد) اسانس به صورت معنی­داری در مقایسه با شاهد کاهش یافت. شاخص مصرف (CI)، نرخ مصرف نسبی (RCR) و نرخ رشد نسبی (RGR) هم در حشرات کامل تیمار شده به­صورت معنی­داری کاهش یافت. شاخص بازدارندگی تغذیه­ای (FDI) 40/59 درصدی تحت تاثیر غلظت کشنده 30 درصد اسانس در حشرات تیمار شده محاسبه شد. با توجه به تاثیر کشندگی و زیرکشندگی مشاهده شده، اسانس E. oleosa می­تواند برای انجام بررسی­های تکمیلی در راستای مدیریت R. dominica مورد توجه قرار گیرد.

کلیدواژه‌ها


عنوان مقاله [English]

Chemical composition, fumigant toxicity and biochemical and anti-nutritional effects of the essential oil of Eucalyptus oleosa Muell. against the lesser grain borer, Rhyzopertha dominica (F.)

نویسنده [English]

  • A. Ebadollahi
Department of Plant Sciences, Moghan College of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
چکیده [English]

The lesser grain borer, R. dominica, causes extensive quantitative and qualitative loses on stored cereal grains. The use of synthetic chemicals in the management of such insect pests resulted in several side effects such as environmental contamination, potential impacts on human health and development of pest resistance. Promising insecticidal potential of the essential oils isolated from several species of Eucalyptus genous against insect pests was shown in recent study. In this study, the lethal and sublethal effects of E. oleosa essential oil were investigaetd against the adults of R. dominica.  Chemical analysis of the essential oil indicated that terpenes, including 1,8-cineole, α-pinene, trans-pinocarveol, (s)-α-terpineol, and β-eudesmol, had high amount. The adult insects were susceptible to the fumigation of essential oil so that LC50 (Lethal Concentration to kill 50% of insects) values were estimated as ‎42.923, ‎35.828, and ‎34.044 µl/l air after 24, 48 and 72 h, respectively. Despite increases in a- and β-estearse enzymes, total protein, glycogen, and lipid contents and digestive amylolytic and proteolytic activities of the adults treated with LC30 (Lethal Concentration to kill 30% of insects) of essential oil were significantly decreased in comparision with the control. The consumption index (CI), relative consumption rate (RCR), and relative growth rate (RGR) were also significantly decreased in treated adults. A 59.40% feeding deterrence index (FDI) was evaluated for insects treated with LC30 of  the essential oil. According to observed lethal and sublethal effects, the essential oil of E. oleosa can be considered in further investigations in the management of R. dominica.

کلیدواژه‌ها [English]

  • Insecticidal properties
  • lethal effects
  • Plant essential oil
  • sublethal effects
  • terpenic copmounds
Abd El-Kareem, S. M. I., El-Sabagh, M. M. M. and El-Banna, A. A. 2022. A comparative ‎study ‎between a commercial mixture compound and its individual active ingredients on the ‎cotton ‎leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) on tomatoes under ‎semi-field ‎conditions. The Journal of Basic and Applied Zoology 83: 23.
Ahmad, F., Ridley, A., Daglish, G.J., Burrill, P.R. and Walter, G. H. 2013. Response of Tribolium castaneum and Rhyzopertha dominica tovarious resources, near and far from grain storage. Journal of Applied Entomology 137: 773–781.
Ahmed, R. and Freed, S. 2021. Biochemical resistance mechanisms against ‎chlorpyrifos, ‎imidacloprid and lambda-cyhalothrin in Rhynchophorus ferrugineus (Olivier) ‎‎(Coleoptera: ‎Curculionidae). Crop Protection 143: 105568.
Aref, S. P., Valizadegan, O. and Farashiani, M. E. 2015. Eucalyptus dundasii Maiden essential ‎oil, chemical composition and insecticidal values against Rhyzopertha dominica (F.) and ‎Oryzaephilus surinamensis (L.). Journal of Plant Protection Research 55: 35–41.
Aref, S. P., Valizadegan, O. and Farashiani, M. E. 2016. The insecticidal effect of essential oil of ‎Eucalyptus floribundi against two ‎major stored product insect pests; Rhyzopertha dominica (F.) ‎and Oryzaephilus surinamensis (L.). Journal of Essential Oil Bearing Plants 19: ‎‎820–831.
Arrese, E. L. and Soulages, J. L. 2010. Insect fat body: energy, metabolism, and regulation. Annual Reviw of Entomology 55: 207–225.‎
Ben-Marzoug, H. N., Romdhane, M., Lebrihi, A., Mathieu, F., Couderc, F., Abderraba, M., Khouja, M. L. and Bouajila, J. 2011. Eucalyptus oleosa essential oils: Chemical composition and antimicrobial and antioxidant activities of the oils from different plant parts (stems, leaves, flowers and fruits). Molecules 16: 1695-1709.
Ben-Marzoug, N. H., Bouajila, J., Ennajar, M., Lebrihi, A., Mathieu, F., Couderc, F.,
Abderraba, M. and Romdhane, M.
2010. Eucalyptus (gracilis, oleosa, salubris and salmonophloia) essential oils: Their chemical composition and antioxidant and antimicrobial activities. Journal of Medicinal Food 13: 1005-1012.
Bernfeld, P. 1955. Amylase, α and β. Methods in Enzymology 1: 149–158.
Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248–254.
Chowanski, S., Lubawy, J., Spochacz, M., Ewelina, P., Grzegorz, S., Rosinski, G. and Slocinska, M. ‎2015. Cold induced changes in lipid, protein and carbohydrate levels in the tropical insect ‎Gromphadorhina coquereliana. Comparative Biochemistry and Physiology: Part A, Molecular & Integrative Physiology183: 57–63.
Collins, P. J., Falk, M. G., Nayak, M. K., Emery, R. N. and Holloway, J. C. 2017. Monitoring ‎resistance to phosphine in the lesser grain borer, Rhyzopertha dominica, in Australia: A ‎national analysis of trends, storage types and geography in relation to resistance detections. ‎Journal of Stored Products Research 70: 25–36.‎
Coppen, J. W. 2002. Eucalyptus the Genus Eucalyptus. Taylor and Francis Inc.
do Nascimento, A. F., da Camara, C. A. G. and de Moraes, M. M. 2018. Fumigant activity of Schinus terebinthifolius essential oil and its selected constituents against Rhyzopertha dominica. Revista Facultad Nacional de Agronomía Medellín 71: 8359–8366.
Ebadollahi, A. and Setzer, W. N. 2020. Evaluation of the toxicity of Satureja intermedia C. A. Mey essential oil to storage and greenhouse insect pests and a predator ladybird. Foods 9: 712.
Ebadollahi, A., Jalali Sendi, J., Maroufpoor, M. and Rahimi-Nasrabadi, M. 2017. Acaricidal potentials of the terpene-rich essential oils of two Iranian Eucalyptus species against Tetranychus urticae Koch. Journal of Oleo Science 66: 307–314.
Ebadollahi, A., Naseri, B., Abedi, Z., Setzer, W. N. and Changbunjong, T. 2022a. Promising insecticidal efficiency of essential oils isolated from four cultivated Eucalyptus species in Iran against the lesser grain borer, Rhyzopertha dominica (F.). Insects 13: 517.
Ebadollahi, A., Naseri, B., Abedi, Z. and Setzer,W. N. 2022b. Chemical profiles and insecticidal potential of essential oils isolated from four Thymus species against Rhyzopertha dominica (F.). Plants 11: 1567.
Ebadollahi, A., Ziaee, M. and Palla, F. 2020. Essential oils extracted from deferent species of the Lamiaceae plant family as prospective bioagents against several detrimental pests. Molecules 25: 1556.
Elpidina, E. N., Vinokurov, K. S., Gromenko, V. A., Rudenshaya, Y. A., Dunaevsky, Y. E. and Zhuzhikov, D. P. 2001. Compartmentalization of proteinases and amylases in Nauphoeta cinerea midgut. Archives of Insect Biochemistry and Physiology 48: 206–216.
Farahani, S., Bandani, A. R. and Amiri, A. 2020. Toxicity and repellency effects of three essential oils on two populations of Tetranychus urticae (Acari: Tetranychidae). Persian Journal of Acarology 9: 67–81.
Filomeno, C. A., Almeida Barbosa, L. C., Teixeira, R. R., Pinheiro, A. L., de Sá Farias, E., Ferreira, J. S. and Picanço, M. C. 2020. Chemical diversity of essential oils of Myrtaceae species and their insecticidal activity against Rhyzopertha dominica. Crop Protection 137: 105309.
Franco, O. L., Rigden, D. J. and Melo, F. R. 2002. Plant α-amylase inhibitors and their interaction with insect α-amylase: Structure, function and potential for crop production. Europen Journal of Biochemitry 269: 397–412.
Hagstrum, D. W. and Athanassiou, C. G. 2019. Improving stored product insect pest management: From ‎theory to practice. Insects 10: 332.
Hemingway, J. and Karunaratne, S. H. P. P. 1998. Mosquito carboxylesterases: A review ‎of ‎the molecular biology and biochemistry of a major insecticide resistance mechanism. Medical and Veterinary Entomology 12: 1–12.
Hernández-Carlos, B. and Gamboa-Angulo, M. 2019. Insecticidal and nematicidal contributions of Mexican flora in the search for safer biopesticides. Molecules 24: 897.
Isman, M. B. 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology 51: 45–66.
Isman, M. B. 2020. Commercial development of plant essential oils and their constituents as active ‎ingredients in bioinsecticides. Phytochemical Review 19: 235–241.
Isman, M. B. and Grieneisen, M. L. 2014. Botanical insecticide research: Many publications, limited useful data. Trends in Plant Sciences 19: 140–145.
Isman, M. B., Wilson, J. A. and Bradbury, R. 2008. Insecticidal activities of commercial rosemary oils (Rosmarinus officinalis.) against larvae of Pseudaletia unipuncta. and Trichoplusia ni. in relation to their chemical compositions. Pharmaceutical Biology 46: 82–87.
Jaimand, K., Rezaee, M. B. and Nadery Hajee Bagher Kandy, M. 2009. Volatile oil constituents of the Eucalyptus viridis R. T. Baker and Eucalyptus oleosa F. Muell. leaves from Iran. Journal of Medicinal Plants 8: 105–108. 
Jankowska, M., Rogalska, J., Wyszkowska, J. and Stankiewicz, M. 2018. Molecular targets for components of ‎essential oils in the insect nervous system—A Review. Molecules 23: 34.‎
Jayakumar, M., Ramachandran, M., Krishnaveni, T. and Nattudurai, G. 2021. Toxicity and biochemical effects of essential oils of Anethum graveolens L. and Melaleuca cajuputi Powell against Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae). International Journal of Tropical Insect Science 41: 945–951.
Khosravi, R., Jalali Sendi, J. and Ghadamyari, M. 2010. Effect of Artemisia annua L. on ‎deterrence and nutritional efficiency of lesser mulberry pyralid (Glyphodes pyloalis Walker) ‎‎(Lepidoptera: Pyralidae). Journal of Plant Protection Research 50: 423–428.‎
Loddé, B., Lucas, D., Letort, J. M., Jegaden, D., Pougnet, R. and Dewitte, J. D. 2015. Acute ‎phosphine poisoning on board a bulk carrier: analysis of factors leading to a fatal case. Journal of Occupational Medicine and Toxicology 10: 10.
Magierowicz, K., Górska-Drabik, E. and Sempruch, C. 2020. The effect of Tanacetum vulgare essential oil ‎and its main components on some ecological and physiological parameters of Acrobasis advenella ‎‎(Zinck.) (Lepidoptera: Pyralidae). Pesticide Biochemistry and Physiology 162: 105–112.‎
Moghaddam, M. and Mehdizadeh, L. 2017. Chemistry of essential oils and factors influencing their ‎constituents. In Handbook of Food Bioengineering, 1st ed.; Grumezescu, A. M. and Holban, A. M. Eds.; ‎ Academic Press: New York, USA, pp 379–419.
Mossa, A. T. H. 2016. Green pesticides: Essential oils as biopesticides in insect- pest management. Journal of Environmental Science and Technology 9: 354–378.
Naseri B. and Majd-Marani S. 2022. Different cereal grains affect demographic traits and digestive enzyme activity of Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae). Journal of Stored Products Research 95: 101898.
Obretenchev, D., Zidan, F. and Atanasova, D. 2020. Food specialization of the lesser grain borer, Rhyzopertha Dominica F. (Coleoptera: Bostrichidae). Canadian Journal of Agriculture and Crops 5: 52–58.
Oftadeh, M., Sendi, J. J., Ebadollahi, A., Setzer, W. N. and Krutmuang, P. 2021. Mulberry protection through flowering-stage essential oil of Artemisia annua against the lesser mulberry pyralid, Glyphodes pyloalisWalker. Foods 10: 210.
Park, M. G., Choi, J., Hong, Y. S., Park, C. G., Kim, B. G., Lee, S. Y., Lim, H. J., Mo H. H., Lim, E. and Cha, W. 2020. Negative effect of methyl bromide fumigation work on the central ‎nervous ‎system. PLoS ONE 15: e0236694.
Pavela, R. and Benelli, G. 2016. Essential oils as ecofriendly biopesticides? Challenges and constraints. Trends in Plant Sciences 21: 1000–1007.
Pichersky, E. and Gershenzon, J. 2002. The formation and function of plantvolatiles: Perfumes for pollinator attraction and defense. Current Opinion in Plant Biology 5: 237–243.
Rahimi-Nasrabadi, M., Pourmortazavi, S. M., Nazarian, S., Ahmadi, F. and
Batooli, H.
2013. Chemical composition, antioxidant, and antibacterial activities of
the essential oil and methanol extracts of Eucalyptus oleosa leaves. International Journal of Food Properties 16: 1080-1090.
Rozman, V., Kalinovic, I. and Korunic, Z. 2007. Toxicity of naturally occurring compounds of ‎Lamiaceae and Lauraceae to three stored-product insects. Journal of Stored Product Research 43: 349–‎‎355.
Sakka, M. K., Riga, M., Ioannidis, P., Baliota, G. V., Tselika, M., Jagadeesan, R., Nayak, M. K., Vontas, J. and Athanassiou, C. G. 2021. Transcriptomic analysis of s-methoprene resistance ‎in the lesser grain borer, Rhyzopertha dominica, and evaluation of piperonyl butoxide as a ‎resistance breaker. BMC Genomics 22: 65.
Sayada, N., Tine, S. and Soltani, N. 2021. Toxicity and physiological effects of essential oil from Lavandula angustifolia (M.) against Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) adults. Journal of Entomological Reseach 45: 929–936.
Scheff, D. S., Campbell, J. F. and Arthur, F. H. 2021.Seasonal, landscape, and attractant effects on lesser ‎grain borer, Rhyzopertha dominica (F.), captures in Northeast Kansas. Agronomy 12: 99.
Senthil-Nathan, S. 2013. Physiological and biochemical effect of neem and other Meliaceae ‎plants secondary metabolites against Lepidopteran insects. Frontiers in Physiology 4: 359.
Shahriari, M., Zibaee, A., Shamakhi, L., Sahebzadeh, N., Naseri, D. and Hoda, H. 2020. Bio-‎efficacy and physiological effects of Eucalyptus globulus and Allium sativum essential oils against ‎Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Toxin Reviews 39: 422–433.
Tine, S., Sayada, N., Tine-Djebbar, F. and Soltani, N. 2021. Recent Advances in Environmental Science from the Euro-Mediterranean and Surrounding Regions. Springer: Cham.
Valcárcel, F., Olmeda, A. S., González, M. G., Andrés, M. F., Navarro-Rocha, J. and González-Coloma, A. 2021. Acaricidal and insect antifeedant effects of essential oils from selected ‎aromatic plants and their main components. Frontiers in Agronomy 3: 662802.
van Asperen, K. 1962. A study of housefly esterases by means of a sensitive colorimetric ‎method.‎‏ ‏Journal of Insect Physiology 8: 401–416.‎
van Handel, E. 1985. Rapid determination of total lipids in mosquitoes. Journal of the American Mosquito Control Association 1: 302–304.
Waldbauer, G. P. 1968. The consumption and utilization of food by insects. Advances in Insect Physiology 5: 229–288.
Yazdani, E., Jalali Sendi, J. and Hajizadeh, J. 2014. Effect of Thymus vulgaris L. and Origanumvulgare L. essential oils on toxicity, food consumption, and biochemical properties of lesser ‎mulberry pyralid Glyphodes pyloalis Walker (Lepidoptera: Pyralidae). Journal of Plant Protection Research 54: 53–61.
Yuval, B., Kaspi, R., Shloush, S. and Warburg, M. S. 1998. Nutritional reserves regulate male participation in Mediterranean fruit fly leks. Ecological Entomology 23: 211–215.