اثر مقادیر مختلف تخم بید آرد Anagasta kuehniellaو مقدار تغذیه مجدد بستر پرورش بر تولید لارو آن در شرایط انسکتاریوم

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه حشره شناسی، دانشکده علوم کشاورزی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران

چکیده

بید آرد  Anagasta kuehniella (Zeller)یکی از حشرات میزبانی است که از آن برای پرورش انبوه زنبور پارازیتوئید براکون (Habrobracon hebetor Say) استفاده می­شود. در این تحقیق، اثر پنج مقدار تخم بید آرد (4/0، 5/0،  6/0، 7/0 و 8/0 گرم) روی فرمول غذایی ثابت (5/1 کیلوگرم آرد گندم و 5/0 کیلوگرم سبوس برنج) برای تمام تیمارها و سپس، اثر افزودن پنج میزان متفاوت از آرد گندم به عنوان تغذیه ثانویه در میانه دوره پرورش (0، 250، 500، 750 و 1000 گرم) بر برخی ویژگی­های مورفولوژیک لارو و نسبت جنسی و باروری حشرات کامل آن مورد ارزیابی قرار گرفت. نتایج نشان داد بیشترین میانگین وزن 100 لارو (35/3 گرم) در حالتی مشاهده شد که کمترین میزان تخم (4/0 گرم) در محیط پرورش اولیه و بیشترین وزن آرد در تغذیه ثانویه (1000 گرم) استفاده شد؛ در حالی که بیشترین وزن کل لارو در یک تیمار (254 گرم)، مربوط به تیمار 8/0 گرم وزن تخم و 1000 گرم آرد افزوده­شده در تغذیه مجدد بود. همچنین، بالاترین نسبت جنسی ماده به نر (29/1 : 1) در تیمار 5/0 گرم تخم و بدون آرد افزوده­شده در تغذیه مجدد مشاهده شد. از طرف دیگر، لاروهای بزرگتر حاصل از پرورش روی محیط­ دارای منابع غذایی بیشتر، پس از تبدیل شدن به حشره کامل نسبت به سایر تیمارها به میزان بیشتری تخم گذاشته و وزن این تخم­ها نیز بیشتر بود. با توجه به نتایج، پیشنهاد می­شود که برای کسب بهترین نتیجه در پرورش  لارو بید آرد در انسکتاریوم، برای شروع پرورش با 2 کیلوگرم بستر پرورش، 5/0-4/0 گرم تخم حشره استفاده شود و در میان دوره به میزان حدود 1-8/0 کیلوگرم آرد گندم به محیط کشت اضافه شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of different amount of flour moth, Anagasta kuehniella eggs and second feeding on its larva production in insectarium condition

نویسندگان [English]

  • M. Mirhoseini Moghadam
  • S. Hesami
Department of Entomology, Faculty of Agricultural Sciences, Islamic Azad University, Shiraz, Iran
چکیده [English]

Mediterranean flour moth Anagasta kuehniella (Zeller) is considered as one of the hosts that used for mass rearing of Habrobracon hebetor Say (Hym.: Braconidae). In this research effects of five amounts of flour moth eggs (0.4, 0.5, 6.0, 0.7 and 0.8 gr) on a constant diet (1.5 kg of wheat flour and 0.5 kg of rice bran) and five amount of wheat flour that added as second feeding at the mid of rearing period (0, 0.25, 0.5 0.75 and 1 kg) were investigated on some larval morphological characteristics and sex ratio and fertility of adults moths. Results showed that maximum weight of 100 larvae (3.35 gr), was obtained in minimum amount of egg weight (0.4 gr) and maximum secondary feeding (1 kg). While, maximum total larvae weight in a treatment (254 gr), was recorded in 0.8 gr egg weight and 1 kg flour weight as secondary feeding. Also highest female/male sex ratio (1.29: 1) observed in 0.5 gr egg weight and zero secondary feeding. On the other hand, bigger larvae that reared on higher amount of flour, laid higher amount of eggs and their eggs were heavier too. According to results, for the best outcome in flour moth rearing in insectarium it would be suggested that at the beginning 0.4-0.5 gr of insect eggs should be added to 2 kg of rearing media and after that 0.8-1 kg of wheat flour should be added to this diet at the mid of rearing period.

کلیدواژه‌ها [English]

  • Female Fertility
  • Flour Moth Rearing
  • Optimum Nutrition
  • Secondary Feeding
  • Sex ratio
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