بررسی مقاومت جمعیت‌های مختلف Trialeurodes vaporariorum (Hem.:Aleyrodidae) به ایمیداکلوپرید و تاثیر آن بر مقاومت Encarsia formosa (Hym.: Aphelinidae)

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه گیاه‌پزشکی پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

چکیده

سفیدبالک گلخانه، Trialeurodes vaporarium (westwood) یکی از مهم­ترین آفات گیاهی در ایران و جهان می­باشد. آفت­کش ایمیداکلوپرید نقش کلیدی را در کنترل این آفت بازی می­کند. در این پژوهش مقاومت جمعیت­های مختلفی از سفیدبالک گلخانه نسبت به ایمیداکلوپرید ((Confidor® SC35% و مقاومت تقاطعی نسبت به تیامتوکسام+لامبداسای­هالوترین و تیاکلوپرید+دلتامترین و تاثیر مقاومت به ایمیداکلوپرید بر مقاومت پارازیتوئید Encarsia formosa Gahan مورد ارزیابی قرار گرفت. سیزده جمعیت سفیدبالک گلخانه از مناطق مختلف کشور جمع­آوری و مورد آزمون قرار گرفتند. سفیدبالک­های جمع­آوری شده روی گیاه خیار رقم سوپر دومینوس در شرایط آزمایشگاهی در دمای ˚C25±2، رطوبت نسبی 10±70 درصد و دوره نوری 16 ساعت روشنایی و 8 ساعت تاریکی پرورش داده شدند. زیست­سنجی به روش غوطه­وری برگ در محلول آفت­کش با 20 پوره سن دوم در هر تکرار انجام شد. بیشترین مقدار LC50 برای جمعیت VGR به میزان 2/856 میلی­گرم بر لیتر به دست آمد. توسعه مقاومت در تمام جمعیت­های مورد آزمایش مشاهده شد و فاکتور مقاومت از 54/2 تا 3/44 متغیر بود. همچنین، مقاومت تقاطعی نسبت به تیامتوکسام+لامبداسای­هالوترین وتیاکلوپرید+دلتامترین مشاهده شد. مقاومت سفیدبالک روی مقاومت پارازیتوئید تاثیری نداشت و تفاوت معنی­داری بین LC50 پارازیتوئید جمع­آوری­شده از مزرعه و پارازیتوئید پرورش یافته روی مقاوم­ترین سفیدبالک و جمعیت حساس مشاهده نشد. مقدار LC50 به دست آمده  به­ترتیب 7/388، 7/324 و 7/317 میلی­گرم بر لیتر بود. نتایج حاصل نشان­دهنده افزایش مقاومت این آفت نسبت به ایمیداکلوپرید و لزوم اتخاذ تدابیر لازم و اعمال مدیریت جهت جلوگیری از گسترش مقاومت می­باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Survey on different populations of Trialeurodes vaporariorum (Hem.: Aleyrodidae) resistance to imidacloprid and its effect on Encarsia formosa (Hym.: Aphelinidae) resistance

نویسندگان [English]

  • Z. Bigham
  • H. Allahyari
  • K. Talebi Jahromi
  • V. Hosseininaveh
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

The greenhouse whitefly, Trialeurodes vaporarium (Westwood), is an invasive insect pest of agriculture and horticulture worldwide. Imidacloprid as chemical pesticide has been used as a key pesticide for controlling sap-sucking pests and play a major role in controlling this pest. The main purpose of this study was investigation of resistance status of T. vaporarium to imidacloprid SC 35% (Confidor®) and cross resistance to Eforia® and Proteus® and effect of imidacloprid resistance on Encarsia formosa Gahan. Thirteen greenhouse whitefly populations were collected from greenhouses and fields and used for tests. Collected populations reared on cucumber plants under laboratory conditions at 25±2˚C, 70±10 % relative humidity and a photoperiod of 16:8 h (L: D). The LC50 values were estimated on the second instar nymphs for all populations using leaf dipping bioassay method. The VGR population showed highest LC50 that was 856.2 mg/L. The development of resistance to imidacloprid was confirmed for all tested populations with resistance factors ranging from 2.54 to 44.3. The most resistant strain showed cross resistance to Eforia® and Proteus®. There was no significant difference between LC50 values of E. formosa populations that collected form field, reared on the most resistant population and sensitive population of T. vaporarium. The LC50s were 388.7, 324.7 and 317.7, respectively. We discuss our results in regard to the evolve of imidacloprid resistance in T. vaporariorum populations and its implications for whitefly control.

کلیدواژه‌ها [English]

  • Cross resistance
  • Bioassay
  • Resistance factor
  • Whitefly
  • Parasitoid
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