بررسی مقاومت جمعیت‌های مختلف Trialeurodes vaporariorum (Hem.:Aleyrodidae) به ایمیداکلوپرید و تاثیر آن بر مقاومت Encarsia formosa (Hym.: Aphelinidae)

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه گیاه‌پزشکی پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

10.22124/iprj.2021.5257

چکیده

سفیدبالک گلخانه، Trialeurodes vaporarium (westwood) یکی از مهم­ترین آفات گیاهی در ایران و جهان می­باشد. آفت­کش ایمیداکلوپرید نقش کلیدی را در کنترل این آفت بازی می­کند. در این پژوهش مقاومت جمعیت­های مختلفی از سفیدبالک گلخانه نسبت به ایمیداکلوپرید ((Confidor® SC35% و مقاومت تقاطعی نسبت به تیامتوکسام+لامبداسای­هالوترین و تیاکلوپرید+دلتامترین و تاثیر مقاومت به ایمیداکلوپرید بر مقاومت پارازیتوئید Encarsia formosa Gahan مورد ارزیابی قرار گرفت. سیزده جمعیت سفیدبالک گلخانه از مناطق مختلف کشور جمع­آوری و مورد آزمون قرار گرفتند. سفیدبالک­های جمع­آوری شده روی گیاه خیار رقم سوپر دومینوس در شرایط آزمایشگاهی در دمای ˚C25±2، رطوبت نسبی 10±70 درصد و دوره نوری 16 ساعت روشنایی و 8 ساعت تاریکی پرورش داده شدند. زیست­سنجی به روش غوطه­وری برگ در محلول آفت­کش با 20 پوره سن دوم در هر تکرار انجام شد. بیشترین مقدار LC50 برای جمعیت VGR به میزان 2/856 میلی­گرم بر لیتر به دست آمد. توسعه مقاومت در تمام جمعیت­های مورد آزمایش مشاهده شد و فاکتور مقاومت از 54/2 تا 3/44 متغیر بود. همچنین، مقاومت تقاطعی نسبت به تیامتوکسام+لامبداسای­هالوترین وتیاکلوپرید+دلتامترین مشاهده شد. مقاومت سفیدبالک روی مقاومت پارازیتوئید تاثیری نداشت و تفاوت معنی­داری بین LC50 پارازیتوئید جمع­آوری­شده از مزرعه و پارازیتوئید پرورش یافته روی مقاوم­ترین سفیدبالک و جمعیت حساس مشاهده نشد. مقدار LC50 به دست آمده  به­ترتیب 7/388، 7/324 و 7/317 میلی­گرم بر لیتر بود. نتایج حاصل نشان­دهنده افزایش مقاومت این آفت نسبت به ایمیداکلوپرید و لزوم اتخاذ تدابیر لازم و اعمال مدیریت جهت جلوگیری از گسترش مقاومت می­باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Survey on different populations of Trialeurodes vaporariorum (Hem.: Aleyrodidae) resistance to imidacloprid and its effect on Encarsia formosa (Hym.: Aphelinidae) resistance

نویسندگان [English]

  • Z. Bigham
  • H. Allahyari
  • K. Talebi Jahromi
  • V. Hosseininaveh
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

The greenhouse whitefly, Trialeurodes vaporarium (Westwood), is an invasive insect pest of agriculture and horticulture worldwide. Imidacloprid as chemical pesticide has been used as a key pesticide for controlling sap-sucking pests and play a major role in controlling this pest. The main purpose of this study was investigation of resistance status of T. vaporarium to imidacloprid SC 35% (Confidor®) and cross resistance to Eforia® and Proteus® and effect of imidacloprid resistance on Encarsia formosa Gahan. Thirteen greenhouse whitefly populations were collected from greenhouses and fields and used for tests. Collected populations reared on cucumber plants under laboratory conditions at 25±2˚C, 70±10 % relative humidity and a photoperiod of 16:8 h (L: D). The LC50 values were estimated on the second instar nymphs for all populations using leaf dipping bioassay method. The VGR population showed highest LC50 that was 856.2 mg/L. The development of resistance to imidacloprid was confirmed for all tested populations with resistance factors ranging from 2.54 to 44.3. The most resistant strain showed cross resistance to Eforia® and Proteus®. There was no significant difference between LC50 values of E. formosa populations that collected form field, reared on the most resistant population and sensitive population of T. vaporarium. The LC50s were 388.7, 324.7 and 317.7, respectively. We discuss our results in regard to the evolve of imidacloprid resistance in T. vaporariorum populations and its implications for whitefly control.

کلیدواژه‌ها [English]

  • Cross resistance
  • Bioassay
  • Resistance factor
  • Whitefly
  • Parasitoid
Abbas, N., Mansoor, M. M., Shad, S. A., Pathan, A. K., Waheed, A., Ejaz, M., Razaq, M. and Zulfiqar, M. A. 2014. Fitness cost and realized heritability of resistance to spinosad in Chrysoperl acarnea (Neuroptera: Chrysopidae). Bulletin of Entomological Research 104(6): 707-715.
Abd-Ella, A. A. 2015. Susceptibility of the pomegranate whitefly, Siphoninus phillyreae (Haliday) (Homoptera: Aleyrodidae) and its parasitoid, Encarsia inaron (Walker) (Hymenoptera: Aphelinidae) to certain insecticides under laboratory conditions. Egyptian Journal of Biological Pest Control 25(3): 689-695.
Almasi, A., Sabahi, Q., Talebi, Kh. and Mardani, A. 2013. Laboratory evaluation of the toxicity of proteus, pymetrozine, deltametrin and pirimicarb on lady beetle Hipodamia variegate (Goeze) (Col.; Coccinelidae). Journal of Plant Protection Research 53(2): 143-147.
Asogwa, E. U., Okelana, F. A., Mokwuny, I. U. Anikwe, J. Ch. And Ndubuaki, T. Ch. N. 2011. Evaluation of new insecticide (Proteus 170 O-TEQ) for the control of the brown cocoa mired (Sahlbergella singularis) in Nigeria. Journal of Life Sciences 5: 393-399.
Balanza, V., Mendoza, J. E. and Bielza, P. 2019. Variation in susceptibility and selection for resistance to imidacloprid and thiamethoxam in Mediterranean populations of Orius laevigatus. Entomologia Experimental et Applicata. 167(7): 626-635.
Basij, M., Talebi, K., Ghadamyari, M., Hosseininaveh, V. and Salami, S. A. 2016. Status of Resistance of Bemisia tabaci (Hemiptera: Aleyrodidae) to neonicotinoids in Iran and detoxification by cytochrome P450-dependent monooxygenases. Neotropical Entomology 46(1): 115-124.
Basit, M., Saeed, S., Ahmad, M. and Sayyed, A. H. 2013. Can resistance in Bemisia tabaci (Homoptera: Aleyrodidae) be overcome with mixtures of neonicotinoids and insect growth regulators? Crop Protection 44: 135–141.
Bass, C., Denholm, I., Williamson, M. S. and Nauen, R. 2015. The global status of insect resistance to neonicotinoid insecticide. Pesticide Biochemistry and Physiology 121: 78–87.
Bi, J. L., Toscano, N. C. and Ballmer G. R. 2002.Greenhouse and field evaluation of six novel insecticides against the greenhouse whitefly Trialeurodes vaporariorum on strawberries. Crop Protection 21: 49-55.
Bi, J. L. and Toscano, N. C. 2007. Current status of the greenhouse whitefly, Trialeurodes vaporariorum, susceptibility to neonicotinoid and conventional insecticides on strawberries in southern California. Pest Management Science 63(8): 747-752.
CABI. 2017. Center for Agriculture and Biosciences International, Invasive Species Compendium. Bemisia tabaci (tobacco whitefly). Fromhttp://www.cabi.org/isc/datasheet/8927.
Bouharroud, R., Hanafi, A., Brown, J. K. and Serghinim M. A. 2006. Resistance and cross-resistance of Bemisia tabaci to three commonly used insecticides in the tomato greenhouse of the Souss valley of Morocco. European Journal of Scientific Research 14(2): 587-594.
Cahill, M., Gorman, K., Day, S., Denholm, I., Elbert, A. and Nauen, R. 1996.Baseline determination and detection of resistance to imidacloprid in Bemisia tabaci (Homoptera: Aleyrodidae). Bulletin of Entomological Research 86: 343-349.
Capinera, J. L. 2008.Greenhouse whitefly, Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae). In: Capinera JL (ed) Encyclopedia of entomology. Springer, Dordrecht, pp. 1723-1726.
Croft, B. A. and Meyer, R. H. 1973. Carbamate and organophosphorus resistance patterns in populations of Amblyseius fallacies. Environmental Entomology 2: 691–695.
Cuthbertson, A. G. S., Buxton, J. H., Blackburn, L. F., Mathers, J. J., Robinson, K. A., Powell, M. E., Fleming, D. A. and Bell, H. A. 2012. Eradicating Bemisia tabaci Q biotype on poinsettia plants in the UK. Crop Protection 42: 42–48.
Delorme, R., Berthier, A. and Auge, D. 1985. The toxicity of two pyrethroids to Encarsia formosa and its host Trialeurodes vaporariorum; Prospecting for a resistant strain of the parasite. Pesticide Science 16: 332-336.
Elbert, A., Haas, M., Springer, B., Thielert, W. and Nauen, R. 2008. Applied aspects of neonicotinoid uses in crop protection. Pest Management Science 64(11): 1099-1105.
Erdogan, C., SibelVelioglu, A., Gurkan, M. O., Denholm, I, and Moores, G. D. 2021. Detection of resistance to pyrothroid and neonicotinoid insecticide in the greenhouse whitefly, Trialeurodes vaporariorum (Hemiptera: Aleyrodidae). Crop protection 146: 105661.
Ghahari, H., Abd-Rabou, S., Zahradnik, J. and Ostovan, H. 2013. Annotated catalogue of whiteflies (Hemiptera: Sternorrhyncha: Aleyrodidae) from Arasbaran, Northwestern Iran. International Journal of Nematology and Entomology 1: 42-52.
Enkegaard, A. 1993. Encarsia formosa parasitizing the poinsettia-strain of the cotton whitefly Bemisiatabaci on poinsettia: bionomics in relation to temperature. Entomologia Experimentalis et Applicata 69: 125-161.
Jones, D. R. 2003. Plant viruses transmitted by whiteflies. European Journal of Plant Pathology 109: 195-219.
Golmohammadi, G., Hosseinigharalari, A., Fasihi, M. and Arbabtafti, R. 2014. Efficacy of one botanical and three synthetic insecticides against silver leaf whitefly, Bemisia tabaci (Hem.: Aleyrodidae) on cucumber plants in the field. Journal of Crop Protection 3: 435–441.
Gorman, K., Devine, G., Bennison, J., Coussons, P., Punchard, N. and Denholm, I. 2007. Report of resistance to the neonicotinoid insecticide imidacloprid in Trialeurodes vaporariorum (Hemiptera: Aleyrodidae). Pest Management Science ­63: 555-558.                                            
Gorman, K., Hewitt, F., Denholm, I. and Devine, G. J. 2002. New developments in insecticide resistance in the glasshouse whitefly (Trialeurodes vaporariorum) and the two-spotted spider mite (Tetranychus urticae) in the UK. Pest Management Science 58(2): 123-130.
Henter, H. J. and Van Lenteren. J. C. 1996. Variation between laboratory population in the performance of parasitoid Encarsia formosa on tow host species, Bemisia tabaci and Trialeurodes vaporariorum. Entomologia Experimentalis et Applicata 80: 427-434.
Horowitz, A.R., Kontsedalov, T. S. and Ishaaya, T. I. 2004. Dynamics of resistance to the neonicotinoid acetamiprid and thiamethoxam in Bemisia tabaci (Hemiptera: Aleyrodidae). Insecticide Resistance and Resistance Management 97(6): 2015-2056.
He, Z., Liu, Y., Wang, L., Guo, Q., Ali, S. and Chen, X. S. 2018. Risk assessment of two insecticides on Encarsia formosa parasitoid of whitefly Bemisia tabaci. Insects 9: 116-125.
Hosseininaveh, V. and Ghadamyari, M. 2013. Principles and concepts of experimental methods in insect biochemistry, physiology and toxicology. University of Tehran Press. 577 pp.
Hoy, M. A. 1985. Recent advances in genetics and genetic improvement of the Phytoseiidae. Annual Review of Entomology 30: 345–370.
Hu, J. S., Gelman, D. B, and Blackburn, M. B. 2002. Growth and development of Encarsia formosa (Hymenoptera: Aphelinidae) in the greenhouse whitefly, Trialeurodes vaporariorum (Homoptera: Aleyrodidae): effect of host age. Archives of Insect Biochemistry and Physiology 49: 125-136.
Jarrahi, A. and Safavi, S. A. 2015. Effects of pupal treatment with Proteus® and Metarhizium anisopliae sensu lato on functional response of Habrobracon hebetor parasitizing Helicoverpa armigera in an enclosed experiment system. Biocontrol Science and Technology, 26(2): 206-216.
Kapantaidaki, D. E., Sadikoglou, E., Tsakireli, D., Kampanis, V., Stavrakaki, M., Schorn, C., Ilias, A., Riga, M., Tsiamis,G., Nauen, R. Skavids, G. Vontas, J. and Tsagkarakou, A. 2017.Insecticide resistance in Trialeurodes vaporariorum population and novel diagnostics for kdr mutation. Pest Management Science 74(1): 59-69.
Karatolos, N., Denholm, I., Williamson, M., Nauen, R. and Gorman, K. 2010. Incidence and characterization of resistance to neonicotinoid insecticides and pymetrozine in the greenhouse whitefly, Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae). Pest Management Science 66: 1304-1307.
Karimi, J. 1999. Virulence of Beauveria bassiana on Trialeurodes vaporariorum. MSc. thesis. University of Tehran. (In Farsi)
Li, S. J., Xue, X., Ahmed, M. Z., Ren, S. X., Du, Y. Z., Wu, J. H., Cuthbertson, A. G. S. and Qiu, B. L., 2011. Host plants and natural enemies of Bemisia tabaci (Homoptera: Aleyrodidae) in China. Insect Science 18: 101–120.
Liu, Sh., Li, X. and Tang Zh. 2003. Host resistance to an insecticide favors selection of resistance in the parasitoid, Cotesia plutellae (Hymenoptera: Braconidae). Biological Control 28: 137-143.
Liu, T. X., Oetting, R. D. and Buntin, G. D. 1993. Population dynamics and distribution of Trialeurodes vaporariorum and Bemisia tabaci (Homoptera: Aleyrodidae) on poinsettia following applications of three chemical insecticides. Journal of Entomological Science 28(1): 126-135.
Li, Y. Dennehy, T. J., Li, X. and Wigert, M. E. 2000. Susceptibility of Arizona whiteflies to chloronicotinyl insecticides and IGRs: new developments in the 1999 season. Proceedings of the Beltwide Cotton Conferences, pp. 1325-1330.
Longhurst, C, Babcock, J. M., Denholm, I., Gorman, K., Thomas, J. D. and Sparks, T. C. 2013. Cross resistance relationships of the sulfoximine insecticide sulfoxaflor with neonicotinoids and other insecticides in the whiteflies Bemisia tabaci and Trialeurodes vaporariorum. Pest Management Science 69: 809–813.
Malumphy, C. 2003. Protocol for the Diagnosis of Quarantine Organism Bemisia tabaci (Gennadius). Central Science Laboratory: Sand Hutton- UK.
Martin, J. H., Mifsud, D. and Rapisarda, C. 2000. The whiteflies (Hemiptera: Alyrodidae) of Europe and the Mediterranean Basin. Bulletin of Entomological Research 90: 407-448.
Nechols, J. R. and Tauber, M. J. 1977. Age specific interaction between the greenhouse whitefly and Encarsia formosa: influence of host on the parasite’s oviposition and development. Environmental Entomology 6: 143-149.
Nell, H. W., Sevenstervander, L. L. A., Woets, J. and van Lenteren, J. C. 1976. The parasite-host relationship between Encarsia formosa (Hymenoptera: Aphelinidae) and Trialeurodes vaporariorum (Homoptera: Aleyrodidae). II. Selection of host stages for oviposition and feeding by the parasite. Zeitschrift fur Angewandte Entomologie 81: 372-376.
Nikolova, I. М. and Georgieva, N. А. 2018. The effects of a synthetic insecticide and a mineral oil on alfalfa insect pests. Pesticide Phytotoxicity (Belgrade) 33: 221–231.
Pappas, M. L., Migkou, F. and Broufas, G. D. 2013. Incidence of resistance to neonicotinoid insecticides in greenhouse populations of the whitefly, Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) from Greece. Applied Entomology and Zoology 48: 373-378.
Pirmoradi Amozegarfard, N. Sheikhigajan, A., Baniameri, V. and Imani, S. 2011. Evaluation of susceptibility of the first instar nymphs and adults of Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) to neonicotinoid insecticide under laboratory conditions. Journal of Entomological Society of Iran 31(1): 13-24.
Robertson, J. L., Russell, R. M., Preisler, H. K. and Savin, N. E. 2007. Bioassay with arthropods. CRC Press, London.
Roditakis, E., Grispou, M., Morou, E., Kristoffersen, J. B., Roditakis, N., Nauen, R., Vontas, J. and Tsagkarakou, A. 2009.Current status of insecticide resistance in Q biotype Bemisia tabaci populations from Crete. Pest Management Science 65(3): 313-322.
Satar, G., Ulusoy, M. R., Nauen, R. and Dong, K. 2018. Neonicotinoid insecticide resistance among populations of Bemisia tabaci in the Mediterranean region of Turkey. Bulletin of Insectology 71(2): 171-177.
Prabhaker, N., Castle, S. J., Naranjo, S. E., Toscano, N. C. and Morse, J. G. 2011. Compatibility of two systemic neonicotinoids, imidacloprid and thiamethoxam, with various natural enemies of agricultural pests. Journal of Economical Entomology 104: 773–781.
Prabhaker, N., Toscano, N. C., Perring, T.M., Nuessley, G., Kido, K. and Youngman, R. R. 1992. Resistance monitoring of the sweet potato whitefly (Homoptera: Aleyrodidae) in the Imperial Valley of California. Journal of Economical Entomology 85: 1063–1068.
Scott, J. G. 1990. Investigating mechanisms of insecticide resistance: Methods, strategies and pitfalls. In: Roush R. T. and Tabashnik E. (Eds.). Pesticides resistance in arthropods, Chapman and hall, New York and Landon. pp.39- 57.
Singh, V., Sood, A. K. and Hayat, Mohammad. 2017. First record of Encarsia formosa Gahan, 1924 (Hymenoptera: Aphelinidae), a parasitoid of the greenhouse whitefly, Trialeurodes vaporariorum (Westwood, 1856) from India. Oriental Insects 52(3): 313-317.
Sohrabi, F., Shishehbor, P., Saber, M. and Mosaddegh, M. S. 2012. Lethal and sublethal effects of buprofezin and imidacloprid on the whitefly parasitoid Encarsia inaron (Hymenoptera: Aphelinidae). Crop Protection 32: 83-89
Talebi Jahromi, Kh. 2012. Pesticide toxicology. University of Tehran press. 508 pp.
Tomizawa, M. and Casida, J. E. 2005. Neonicotinoid insecticide toxicology: mechanisms of selective action. Annual Review of Pharmacology and Toxicology 45: 247–268.
Van Lenteren, J. C. and Martin, N. A. 2000. Biological control of whiteflies. In: Albajes, R., Gullino, M., van Lenteren, J. C. and Elad, Y. (Eds.). Integrated pest and disease management in greenhouse crops. Kluwer Publishers, Dordrecht, pp. 202–214.
Vassiliou, V., Emmanouilidou, M., Perrakis, A., Morou, E., Vontas, J., Tsagkarakou, A. and Roditakis, E. 2011. Insecticide resistance in Bemisiatabaci from Cyprus. Insect Science 18(1): 30-39.
Walker, P. W. and Turling, D. J. 1984. Insecticide resistance in Encarsia formosa. Proceedings of Brighton Crop Protection Conference on Pests and Diseases. Brighton Metropole, England. pp. 541-546.
Wang, Z., Dai, P., Yang, X., Ruan, Ch., Biondi, A., Desneuxd, N and Zanga, L. 2019. Selectivity of novel and traditional insecticides used for management of whiteflies on the parasitoid Encarsia formosa. Pest Management Science 75: 2716–2724.
Wu, G. and Miyata, T. 2005. Susceptibilities to methamidophos and enzymatic characteristics in 18 species of pest insects and their natural enemies in crucifer vegetable crops. Pesticide Biochemistry and Physiology 82: 79–93.