باکتری Wolbachia و ناسازگاری سیتوپلاسمی در زنبور پارازیتوئید Habrobracon hebetor (Hym.: Braconidae)

نوع مقاله : مقاله پژوهشی

نویسندگان

دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

چکیده

ناسازگاری سیتوپلاسمی (CI) معمول­ترین دستکاری تولیدمثلی القا شده توسط باکتری اجباری درون­سلولی Wolbachia است، که در صورت جفت­گیری نرهای آلوده با ماده غیرآلوده اتفاق می­افتد. حاصل ناسازگاری در گونه­های دیپلوئید، مرگ جنینی است و در گونه­های هاپلودیپلوئید ناسازگاری به دو شکل اتفاق می­ا­فتد: توسعه تخم­های بارور به نر (Male development) و مرگ و میر ماده (Female mortality). زنبور پارازیتوئید Habrobracon hebetor آلوده به استرین القاکننده ناسازگاری باکتری Wolbachia است. در پژوهش حاضر، از زنبور پارازیتوئید Habrobracon hebetor آلوده به استرین القاکننده ناسازگاری باکتری Wolbachia استفاده شده است؛ بدین صورت که پس از حذف Wolbachia با آنتی­بیوتیک، نوع ناسازگاری در این زنبور پارازیتوئید، با مقایسه بین تعداد کل نتاج و تعداد نرهای تولید شده و مرحله­ای که بیشترین مرگ و میر اتفاق می­افتد، در تلاقی سازگار و ناسازگار تعیین شد. همچنین، اثر دما بر شدت بروز ناسازگاری با مقایسه تعداد نتاج در سه دمای 25، 30 و 35 درجه سلسیسوس در دو تلاقی تعیین شد. نتایج حاصل از این پژوهش نشان داد که تعداد کل نتاج در تلاقی ناسازگار در مقایسه با تلاقی سازگار کمتر است، در صورتی که اختلافی در تعداد نرهای تولید شده وجود ندارد. همچنین، بیشترین مرگ و میر مربوط به مرحله­ی تفریخ تخم است. در نهایت، دمای بالا در تلاقی ناسازگار منجر به افزایش تعداد کل نتاج و کاهش شدت ناسازگاری شد. مطابق با نتایج به دست آمده، به نظر می­رسد در این زنبور پارازیتوئید ناسازگاری از نوع مرگ و میر ماده است. این نتایج جزئیات بیشتری از برهمکنش میزبان- Wolbachia را ارائه می­دهد.

کلیدواژه‌ها

عنوان مقاله [English]

Wolbachia and cytoplasmic incompatibility in Habrobracon hebetor (Hym.: Braconidae)

نویسندگان [English]

  • S. F. Nasehi
  • Y. Fathipour
  • M. Mehrabadi
Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
چکیده [English]

Cytoplasmic incompatibility (CI) is the most common reproduction manipulation induced by intracellular bacteria, Wolbachia which occurs when infected males mate with an uninfected female. The result of incompatibility in diploid species is embryonic death and in haplodiploid species, CI occurs in two ways: male development (MD) and female mortality (FM). The parasitoid wasp, Habrobracon hebetor infected with a Wolbachia- CI strain. In the present study, after removal of Wolbachia with antibiotic, the incompatibility type was determined by comparing the number of offspring, the number of male progeny and the mortality rate in different developmental stages in the compatible and incompatible crosses of H. hebetor. Also, the effect of temperature on CI intensity was investigated by comparing the number of offspring at 25, 30 and 35 °C in the compatible and incompatible crosses. The results showed that the number of offspring in the incompatible cross was less than the compatible cross and there was no difference in the number of produced males. Moreover, the highest mortality was observed in the egg hatching stage. Finally, high temperatures increased the number of offspring and decreased the severity of CI in the incompatible cross. Together, it can be concluded that CI type is female mortality in these parasitoid wasps. These results shed more light on the mechanisms underlying Wolbachia- host interaction.

کلیدواژه‌ها [English]

  • Wolbachia
  • cytoplasmic incompatibility
  • female mortality
  • parasitoid wasp

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تحقیقات آفات گیاهی
دوره 11، شماره 2
شهریور 1400
صفحه 53-66

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  • تاریخ دریافت: 30 شهریور 1400
  • تاریخ پذیرش: 30 شهریور 1400

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