تاثیر پیوند بر پارامترهای رویشی گوجه‌فرنگی و القای مقاومت علیه شب‌پره مینوز گوجه‌فرنگی Tuta absoluta (Meyrick) (Lep. : Gelechiidae)

نوع مقاله : مقاله پژوهشی


1 گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه ولی عصر (عج) رفسنجان، رفسنجان، ایران

2 گروه باغبانی، دانشکده کشاورزی، دانشگاه ولی عصر (عج) رفسنجان، رفسنجان، ایران


در این پژوهش، تاثیر پیوند گوجه‌فرنگی روی پایه بادمجان، بر پارامترهای رشدی گیاه گوجه‌فرنگی و پارامترهای زیستی شب‌پره مینوز گوجه‌فرنگی Tuta absoluta (Meyrick) (Lep.: Gelechiidae) بررسی شد. برای بررسی باروری و زادآوری، یک جفت حشره بالغ با سن کمتر از 24 ساعت (نر و ماده) شب‌پره مینوز گوجه فرنگی روی هر یک از گیاهان (پیوندی و غیرپیوندی) مستقر شد. سپس، به منظور اندازه­­گیری دوره رشد جنینی، لاروی و شفیرگی، یک تخم (در 15 تکرار) روی هر گیاه تا زمان ظهور حشرات کامل گذاشته شد. 75 روز بعد از پیوند، پارامترهای رشدی گیاهان پیوندی شامل ارتفاع گیاه، تعداد گره و تعداد برگ نسبت به گیاهان غیرپیوندی دارای افزایش معنی­داری بودند. اما، سطح برگ در گیاه پیوندی کاهش یافت. همچنین، وزن تر و خشک ریشه در گیاهان پیوندی نسبت به گیاهان غیرپیوندی بیشتر بود. طول دوره رشدی لارو و شفیره در حشرات تغذیه کرده با گیاه پیوندی بیشتر از حشرات تغذیه کرده از گیاه غیر پیوندی بود. به­­علاوه، میانگین تعداد تخم­های حشرات کامل ماده شب‌پره مینوز گوجه­فرنگی، روی گیاهان پیوندی کمتر از گیاهان غیر­پیوندی بود. ،مقدار فنل کل در زمان‌های متفاوت (10، 20 و 30 روز) پس از آلودگی با شب‌پره مینوز گوجه‌فرنگی در گیاهان پیوندی نسبت به غیرپیوندی بالاتر بود. نتایج به­دست آمده در این تحقیق به روشنی نشان داد که پیوند گوجه‌فرنگی روی پایه‌ی بادمجان در بهبود رشد گیاه موثر بود و مقاومت آن را نسبت به آفت شب‌پره مینوز گوجه فرنگی افزایش داد.


عنوان مقاله [English]

Effect of grafting on vegetative parameters of tomato and induction of resistance against Tuta absoluta (Meyrick) (Lep.: Gelechiidae)

نویسندگان [English]

  • N. Shariatzadeh Mirhoseini 1
  • S. S. Shahidi Noghabi 1
  • H. R. Karimi 2
1 Department of Crop Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran
2 Department of Horticultural Science, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran
چکیده [English]

In this study, the effect of grafting of tomato on eggplant rootstock were investigated on growth indices of the tomato plant and biological parameters of Tuta absoluta (Meyrick) (Lep.: Gelechiidae). In order to evaluate of fertility and fecundity, a pair of adult insects (male and female) of T. absoluta, with less than 24 h age, were kept on each plant (grafted and non-grafted). Then, one egg (in 15 replications) was placed on each plant to measure the developmental period of embryonic, larval and papal stages until adult insect emergence. Seventy-five days after grafting, except the stem diameter, all growth indices of grafted plants including plant height, number of nodes and leaves were significantly higher than non-grafted plants. Also, weight of fresh and dry root of grafted plants were higher compare to non-grafted plants. Larval and pupal developmental period of T. absoluta fed on grafted plants were significantly higher than non-grafted plants. Besides, the mean fecundity of the adult female of T. absoluta was lower on grafted plants. Amount of total phenol content in different days after infestation with T. absoluta indicated a significant increase in grafted plant. Results of this research clearly showed that the grafting of tomato plants on eggplant rootstocks could be an effective approach for increasing of plant growth and subsequently its resistance to T. absoluta.

کلیدواژه‌ها [English]

  • Grafting
  • tomato leaf miner
  • phenol
  • induced resistance
Abutorabi, E. 2012. Application of grafting method for management of soil borne disease of greenhouse Tomato. Plant Pathology Science 2(2): 1-11. (In Farsi).
Albacete, A., Martínez-Andújar, C., Ghanem, M. E., Acosta, M., Sánchez-Bravo, J., Asins, M. J., Cuartero, J., Lutts, S., Dodd, I. C. and Pérez-Alfocea, F. 2009. Rootstock-mediated changes in xylem ionic and hormonal status are correlated with delayed leaf senescence, and increased leaf area and crop productivity in salinized tomato. Plant, Cell, and Environment 32(7): 928-938.
Alvarez-Hernandez, J. C., Cortez-Madrigal, H., Garcia-Ruiz, I., CEJA-TORRES, L. F. and Perez-Dominguez, J. F. 2009. Incidence of pests in grafts of tomato (Solanum lycopersicum) on wild relatives. Revista Colombiana de Entomología 35(2): 150-155.
Barrett, C. E., Zhao, X. and McSorley, R. 2012. Grafting for root-knot nematode control and yield improvement in organic heirloom tomato production. HortScience 47(5): 614-620.
Biondi, A., Guedes, R. N. C., Wan, F. H. and Desneux, N. (2018). Ecology, worldwide spread, and management of the invasive South American tomato pinworm, Tuta absoluta: past, present, and future. Annual Review of Entomology 63: 239-258.
Cohen, R., Burger, Y., Horev, C., Porat, A. and Edelstein, M. 2005. Performance of Galia‐type melons grafted on to Cucurbita rootstock in Monosporascus cannonballus‐infested and non‐infested soils. Annals of Applied Biology 146(3): 381-387.
Colla, G., Rouphael, Y., Cardarelli, M., Salerno, A. and Rea, E. 2010. The effectiveness of grafting to improve alkalinity tolerance in watermelon. Environmental and Experimental Botany 68(3): 283-291.
Cortez-Madrigal, H. 2010. Resistencia a insectos de tomate injertado en parientes silvestres con énfasis Bactericera cockerelli Sulc. (Hemiptera: Psyllidae). Bioagro 22: 11–16.
Crinò, P., Bianco, C. L., Rouphael, Y., Colla, G., Saccardo, F. and Paratore, A. 2007. Evaluation of rootstock resistance to fusarium wilt and gummy stem blight and effect on yield and quality of a grafted ‘Inodorus’ melon. HortScience 42(3): 521-525.
Di gioia, F., Serio, F., Buttaro, D., Ayala, O. and Santamaria, P. 2010. Influence of rootstock vegetative growth, fruit yield and quality in ‘Cuore di Bue’, an heirloom tomato. Journal of Horticultural Science and Biotechnology 85: 477–482.
Dos Santos, V. L., de Souza Monteiro, A., Braga, D. T. and Santoro, M. M. 2009. Phenol degradation by Aureobasidium pullulans FE13 isolated from industrial effluents. Journal of Hazardous Materials 161(2-3): 1413-1420.
Edelstein, M. 2004. Grafting vegetable-crop plants: Pros and cons. Acta Horticulturae 659: 235-238.
Edelstein, M., Cohen, R., Burger, Y., Shriber, S., Pivonia, S. and Shtienberg, D. 1999. Integrated management of sudden wilt in melons, caused by Monosporascus cannonballus, using grafting and reduced rates of methyl bromide. Plant Disease 83(12): 1142-1145.
Edelstein, M., Tadmor, Y., Abo-Moch, F., Karchi, Z. and Mansour, F. 2000. The potential of Lagenaria rootstock to confer resistance to the carmine spider mite, Tetranychus cinnabarinus (Acari: Tetranychidae) in Cucurbitaceae. Bulletin of Entomological Research 90(2): 113-117.
FAO STAT, 2013.  Production crops. http://faostat.fao.org/site/609/default.aspx#ancor [accessed on 20 December 2013].
Fernandez-Garcia, N., Martínez, V., Cerdá, A. and Carvajal, M. 2004. Fruit quality of grafted tomato plants grown under saline conditions. Journal of Horticultural Science and Biotechnology 79(6): 995-1001.
Flores, F. B., Sanchez-Bel, P., Estan, M. T., Martinez-Rodriguez, M. M., Moyano, E., Morales, B., Compos, J. F., GarciaAbellan, J. O., Egea, M. I., Fernandez-Garcia, N., Romojaro, F. and Bolarin, M. C. 2010. The effectiveness of grafting to improve tomato fruit quality. Scientia Horticulturae 125: 211-217.
Ghaneie, A., Safaie, N., Mehrabi, R. and Sanjarian, F. 2017. The effect of salicylic acid application on the reduction of wheat septoria leaf blotch (STB) symptoms causing by Zymoseptoria tritici. Crop Biotechnology 17: 43-54.
Goreta Ban, S., Dumičić, G., Raspudić, E., Vuletin Selak, G. and Ban, D. 2014. Growth and yield of grafted cucumbers in soil infested with root-knot nematodes. Chilean Journal of Agricultural Research 74(1): 29-34.
Goudriaan, J. and Monteith, J. L. 1990. A mathematical function for crop growth based on light interception and leaf area expansion. Annals of Botany 66(6): 695-701.
He, Y., Zhu, Z., Yang, J., Ni, X. and Zhu, B. 2009. Grafting increases the salt tolerance of tomato by improvement of photosynthesis and enhancement of antioxidant enzymes activity. Environmental and Experimental Botany 66(2): 270-278.
Heuvelink, E. 2005. Tomatoes. CABI Publishing, 339 P.
Hunt, R. 1982. Plant Growth Curves: the Functional Approach to Plant Growth Analysis. Edward Arnold, London, 248 pp.
Ioannou, N. 2001. Integrating soil solarization with grafting on resistant rootstocks for management of soil-borne pathogens of eggplant. Journal of Horticultural Science and Biotechnology 76(4): 396-401.
Kato, T. and Lou, H. 1989. Effects of rootstock on the yield, mineral nutrition and hormone level in xylem sap in eggplant. Journal of the Japanese Society for Horticultural Science 58(2): 345-352.
Lee, J. M., Kubota, C., Tsao, S. J., Bie, Z., Echevarria, P. H., Morra, L. and Oda, M. 2010. Current status of vegetable grafting: Diffusion, grafting techniques, automation. Scientia Horticulturae 127(2): 93-105.
Leogrande, R., Lopedota, O., Montemurro, F., Vitti, C. and Ventrella, D. 2012. Effects of irrigation regime and salinity on soil characteristics and yield of tomato. Italian Journal of Agronomy 8: 50-57.
Louws, F. J., Rivard, C. L., and Kubota, C. 2010. Grafting fruiting vegetables to manage soilborne pathogens, foliar pathogens, arthropods and weeds. Scientia Horticulturae 127(2): 127-146.
Moghbeli Gharaei, A., Estaji, A. and Shahidi Noghabi, S. 2018. Evaluation of some physiological indices of resistance of different varieties of cucumber Cucumis sativus L. against Aphis gossypii. Journal of Greenhouse Culture Science and Technology 9(3): 79-92. (In Farsi).
Mohammed, S. M. T., Humidan, M., Boras, M. and Abdalla, O. A. 2009. Effect of grafting tomato on different rootstocks on growth and productivity under glasshouse conditions. Asian Journal of Agricultural Research 3(2): 47-54.
Niraz, S., Leszczyński, B., Ciepiela, A., Urbańska, A. and Warchol, J. 1985. Biochemical aspects of winter wheat resistance to aphids. International Journal of Tropical Insect Science 6(3): 253-257.
Oda, M., Tsuji, K. and Sasaki, H. 1993. Effect of hypocotyl morphology on survival rate and growth of cucumber [Cucumis sativus] seedlings grafted on Cucurbita spp. Japan Agricultural Research Quarterly 26: 259- 263
Salehi-Mohammadi, R., Khasi, A., Lee, S. G., Huh, Y. C., Lee, J. M. and Delshad, M. 2009. Assessing survival and growth performance of Iranian melon to grafting onto Cucurbita rootstocks. Korean Journal of Horticultural Science and Technology 27(1): 1-6.
Salehi, R., Kashi, A., Lee, J. M., Babalar, M., Delshad, M., Lee, S. G. and Huh, Y. C. 2010. Leaf gas exchanges and mineral ion composition in xylem sap of Iranian melon affected by rootstocks and training methods. HortScience 45(5): 766-770.
Shahabi, M. R., Zakerin, A. R. and Shirzadi, H. 2013. The effect of different grafting techniques on the growth and performance of Lycopersicon esculentum L. var. Redtop. The first electronical national Conference on new topics in horticulture, Jahrom, 1-5 pp. (in Farsi).
Silva, G. A., Picanço, M. C., Bacci, L., Crespo, A. L., Rosado, J. F. and Guedes, R. N. 2011. Control failure likelihood and spatial dependence of insecticide resistance in the tomato pinworm, Tuta absoluta. Pest Management Science 67: 913–920.
Tamoli Torfi, E., Seraj, A. A. and Rajabpour, A. 2015. Host preference of Tomato Leaf miner, Tuta absoluta (Meyrick) (Lep.: Gelechiidae) on Solanaceous Plants. First National Conference on Agriculture, Environment and Food Security, Jiroft, 1-4 pp.
Tamoli Torfi, E., Seraj, A. A. and Rajabpour, A. 2017. Population Parameters of Tomato Leaf miner, Tuta absoluta (Meyrick) (Lep.: Gelechiidae) on Three Solanaceous Plants in Laboratory Condition. Applied Research in Plant Protection 6(2): 83-92.
Todd, G. W., Getahun, A. and Cress, D. C. 1971. Resistance in barley to the greenbug, Schizaphis graminum. Toxicity of phenolic and flavonoid compounds and related substances. Annals of Entomological Society of America 64(3): 718-722.
Turhan, A., Ozmen, N., Serbeci, M. S. and Seniz, V. 2011. Effects of grafting on different rootstocks on tomato fruit yield and quality. Horticultural Science 38(4): 142-149.
Van der Westhuizen, A. J. and Pretorius, Z. 1995. Biochemical and physiological responses of resistant and susceptible wheat to Russian wheat aphid infestation. Cereal Research Communications 23(3): 305-313.
Vinkovic Vrcek, I., Samobor, V., Bojic, M., Medic-Saric, M., Vukobratovic, M., Erhatic, R., Horvat, D. and Matotan, Z. 2011. The effect of grafting on the antioxidant properties of tomato (Solanum lycopersicum L.). Spanish Journal of Agricultural Research 9: 844-851.
Wang, J. W., Zheng, L. P., Wu, J. Y. and Tan, R. X. 2006. Involvement of nitric oxide in oxidative burst, phenylalanine ammonia-lyase activation and Taxol production induced by low-energy ultrasound in Taxus yunnanensis cell suspension cultures. Nitric Oxide 15(4): 351-358.
Wójcicka, A. 2010. Cereal phenolic compounds as biopesticides of cereal aphids. Polish Journal of Environmental Studies 19(6): 1337-1343.
Yamakawa, B. 1983. Grafting. In: Nishi, S. (ed.). Vegetable handbook. Yokendo Book Co., Tokyo. 141–153 pp.
Yetışır, H., Sari, N. and Yücel, S. 2003. Rootstock resistance to Fusarium wilt and effect on watermelon fruit yield and quality. Phytoparasitica 31(2): 163-169.
Žanić, K., Dumičić, G., Urlić, B., Vuletin Selak, G. and Goreta Ban, S. 2017. Bemisia tabaci (Gennadius) population density and pupal size are dependent on rootstock and nitrogen in hydroponic tomato crop. Agricultural and Forest Entomology 19(1): 42-51.