تاثیر حشره کشی اسانس گیاه بادرشبو Dracocephalum moldavica روی زنبور پارازیتوئید Habrobracon hebetor و میزبان های آن Anagasta kuehniella و Plodia interpunctella

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه تولیدات گیاهی، دانشکده ی کشاورزی و منابع طبیعی مغان، دانشگاه محقق اردبیلی، اردبیل، ایران

2 گروه گیاه پزشکی، دانشکده ی کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران.

چکیده

زنبور Habrobracon hebetor Say، پارازیتوئید بیرونی مرحله ­ی لاروی تعداد زیادی از شب ­پره ­ها محسوب می ­شود. در این مطالعه، سمیت اسانس گیاه بادرشبو (Dracocephalum moldavica L.) روی شب ­پره­ ی مدیترانه ­ای آرد (Anagasta kuehniella Zeller)، شب ­پره ­ی هندی (Plodia interpunctella Hübner) و زنبور پارازیتوئید H. hebetor مورد ارزیابی قرار گرفت. اسانس گیاه بادرشبو به روش تقطیر با بخار آب با استفاده از دستگاه کلونجر استخراج شد و ترکیبات شیمیایی آن با استفاده از دستگاه کروماتوگرافی گازی متصل به طیف ­سنج جرمی (GC-MS) شناسایی شدند. Piperitenone Oxide (03/19 درصد)، Piperitone Oxide (74/13 درصد) و Citral (79/9 درصد) به­ عنوان ترکیبات اصلی اسانس بادرشبو می­ باشند. غلظت کشنده­ ی 50 درصد (LC50) اسانس مذکور علیه حشرات کامل زنبور پارازیتوئید H. hebetor و حشرات کامل شب ­پره ­های A. kuehniella و P. interpunctellaبه ترتیب 995/0، 631/9 و 252/10 میکرولیتر بر لیتر هوا به دست آمد. به منظور ارزیابی تاثیر زیرکشندگی، حشرات کامل زنبور پارازیتوئید در معرض غلظت زیرکشنده 25 درصد اسانس گیاه بادرشبو قرار داده شدند و سپس شاخص­ های جمعیت شناسی زنبورهای پارازیتوئید زنده مورد مطالعه قرار گرفتند. نرخ ذاتی افزایش جمعیت (r)، نرخ متناهی افزایش جمعیت (λ)، نرخ خالص تولیدمثل (R0)، نرخ ناخالص تولیدمثلی (GRR) و متوسط زمان یک نسل (T) زنبور پارازیتوئید، به­ طور معنی­ داری تحت تاثیر غلظت مورد مطالعه ­ی اسانس کاهش پیدا کرد. نتایج تحقیق حاضر نشان داد که اسانس گیاه بادرشبو می­ تواند در مدیریت شب ­پره ­ی مدیترانه­ ای آرد و شب­ پره ­ی هندی موثر واقع شود. با این وجود، کاربرد هم­زمان اسانس بادرشبو و زنبور پارازیتوئید H. hebetor جهت مدیریت آفات مذکور، با توجه به سمیت و اثرات دموگرافیکی منفی اسانس روی زنبور پارازیتوئید، پیشنهاد نمی ­شود.  

کلیدواژه‌ها


عنوان مقاله [English]

Insecticidal effects of Moldavian dragonhead, Dracocephalum moldavica, essential oil on the parasitoid wasp Habrobracon hebetor and its hosts Anagasta kuehniella and Plodia interpunctella

نویسندگان [English]

  • A. Ebadollahi 1
  • V. Mahdavi 2
1 Department of Plant Production, Moghan College of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
2 Department of Plant Protection, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
چکیده [English]

Habrobracon hebetor Say is a larval ecto-parasitoid of several moths. In this study, toxicity of Moldavian dragonhead (Dracocephalum moldavica L.)essential oil on Mediterranean flour moth (Anagasta kuehniella Zeller), Indian meal moth (Plodia interpunctella Hubner) and H. hebetor parasitoid waspwere assessed. Essential oil of D. moldavica was extracted by hydrodistillation method using a Clevenger apparatus and its chemical constituents were detected by Gas Chromatography-Mass Spectrometry (GC-MS). Piperitenone Oxide (19.03%), Piperitone Oxide (13.74%) and Citral (9.79%) were identified as the main constituents of the essential oil. It was found that 50% lethal concentration (LC50) values of the essential oil against H. hebetor, A. kuehniella and P. interpunctella adults estimated were 0.995, 9.631 and 10.252 μl/l air, respectively. In order to assess the sub-lethal effects, adult wasps were exposed to LC25 of D. moldavica essential oil and then the demographic parameters of live parasitoids were evaluated. The intrinsic rate of increase (r), finite rate of increase (λ), net reproductive rate (R0), gross reproductive rate (GRR) and mean generation time (T) were significantly decreased by essential oil. Results of the present study indicated that the essential oil of D. moldavica can be effective in the management of A. kuehniella and P. interpunctella. However, simultaneous application of D. moldavica essential oil and the parasitoid wasp H. hebetor in the management of aforementioned pests, based on toxicity and negative demographic effects of essential oil on this parasitoid wasp is not recommended.  

کلیدواژه‌ها [English]

  • Plant essential oils
  • fumigant toxicity
  • sub-lethal effects
  • Habrobracon hebetor
  • Pyralidae
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