تاثیر افزودن پروتئین های کازئین و زئین به رژیم غذایی بر برخی از شاخص‌های تغذیه‌ای و ایمنی‌شناختی Ephestia kuehniella Zeller (Lep.: Pyralidae)

نویسندگان

گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان

چکیده

تخم­ها و لاروهای شب­پره آرد Ephestia kuehniella Zeller (Lep.: Pyralidae)، به­طور وسیع در برنامه­های کنترل بیولوژیک به‌منظور پرورش پارازیتوئیدها و شکارگرها مورد استفاده قرار می­گیرند. در این پژوهش، واکنش­های تغذیه­ای و فعالیت آنزیم­های گوارشی لاروهای سن چهارم همراه با برخی پارامترهای ایمنی­شناسی E. kuehniella روی رژیم غذایی حاوی پروتئین های کازئین و زئین در شرایط آزمایشگاهی بررسی شدند. لاروهای سن چهارم پرورش‌یافته روی رژیم غذایی حاوی پروتئین کازئین بیش­ترین مقدار نرخ مصرف نسبی (2/0±9/5) و شاخص رشد نسبی (3/0±3/11) را دارا بودند. بیش­ترین و کم­ترین فعالیت لیپاز، تریپسین، الاستاز، کربوکسی پپتیداز و آمینو پپتیداز به­ترتیب در لاروهای تغذیه کرده از پروتئین­های کازئین و زئین بود. در بحث ایمنی­شناسی تزریق توئین و Beauveria bassiana (Balsamo) Vuillemin در تیمار با پروتئین کازئین باعث افزایش معنی­دار تعداد کل سلول‌های خونی و تعداد گرانولوسیت­ها و پلاسماتوسیت­ها شد. نتایج تحقیق حاضر نشان داد که ماهیت پروتئین موجود در رژیم غذایی اثرات متفاوتی بر سازوکار فیزیولوژی تغذیه و هم­چنین پاسخ ایمنی دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of casein and zein as additives on some nutritional and immunological indices of Ephestia kuehniella Zeller (Lep: Pyralidae)

نویسندگان [English]

  • M. Ghasemi
  • Jalal Jalali Sendi
  • A. Zibaee
Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan
چکیده [English]

The eggs and larvae of the Mediterranean flour moth, Ephestia kuehniella (Zell.), are widely used to rear parasitoids and predators for biological control programs. In this study, nutritional responses and digestive enzymes activities of the fourth instar larvae, as well as some immunological parameters of E. kuehniella, were studied on the diet containing casein and zein proteins under laboratory conditions. The fourth larval instar reared on diet containing casein had the highest relative rate of consumption (5.9 ± 0.2) and relative growth rate (11.3 ± 0.3). The highest and the lowest activity of lipase, tripcine, elastase, carboxypeptidase and aminopeptidase in larvae fed were on casein and zein proteins. In the immunological study, injection of tween and Beauveria bassiana (Balsamo) Vuillemin in larvae treated by casein protein significantly increased the total number of hemocyte and the number of granulocytes and plasmatocytes. This study indicated that the nature of the protein in the diet effects on physiological mechanisms of feeding and affect the immune response. 

کلیدواژه‌ها [English]

  • Feeding indices
  • Casein
  • Zein
  • Digestive Enzyme
  • Cellular immunity
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