بهینه سازی مصرف حشره کش کلرپایریفوس علیه شب پره هندی Plodia interpunctella ، شب پره مدیترانه ای آرد Anagasta kuehniella و شب پره موم خوار بزرگ Galleria mellonella با روش سطح پاسخ در شرایط آزمایشگاهی

نوع مقاله : مقاله پژوهشی


دانشکده کشاورزی، دانشگاه صنعتی شاهرود، شاهرود


شب ­پره هندی Plodia interpunctella H.، شب ­پره مدیترانه ­ای آرد .Anagasta kuehniella و شب­ پره موم ­خوار بزرگ Galleria mellonella L از آفات بسیار مهم محصولات انباری هستند که به منظور کنترل آن­ ها، به طور معمول از حشره­ کش­ های شیمیایی استفاده می ­شود. استفاده بی ­رویه از حشره­ کش­ ها، مشکلات جدی زیست­محیطی و همچنین عوارض مزمنی روی سلامت انسان ایجاد می ­کند، بنابراین با انتخاب الگوی صحیح و به­ کارگیری روش ­های مناسب می ­توان میزان مصرف حشره ­کش را بهینه کرد. در این پژوهش از روش سطح پاسخ به منظور تعیین نقاط بهینه مصرف حشره­ کش کلرپایریفوس، جهت دستیابی به بیشینه مرگ و میر با کمترین دز مصرفی استفاده شد و تاثیر دما (35-25 درجه سلسیوس)، رطوبت (80-60 %) و دز مصرف (2000-1400 میکرولیتر) بر میزان مرگ و میر لارو سن پنجم سه آفت مهم انباری مورد ارزیابی قرار گرفت. آزمایش ­ها بر اساس طرح مرکب مرکزی، انجام شد. بیشینه مرگ و میر با این روش، 9/7 عدد لارو سن پنج شب ­پره هندی در شرایط دمایی 35 درجه سلسیوس، رطوبت 80 % و دز 1400 میکرولیتر حشره­ کش در 1000 میلی­لیتر آب، 1/7 عدد لارو شب­پره مدیترانه ­ای در دمای 35 درجه سلسیوس، رطوبت 75 % و دز 1400 میکرولیتر حشره­ کش در 1000 میلی­ لیتر آب و برای شب­ پره موم ­خوار بزرگ 9/7 عدد لارو سن پنج در دمای 35 درجه سلسیوس، رطوبت 80 % و دز مصرفی 1500 میکرولیتر حشره­ کش در 1000 میلی لیتر تعیین شد. نتایج آزمایش بیانگر تاثیر خطی و درجه دوم معنی­دار دز مصرفی و درجه حرارت روی مرگ و میر لارو سن پنج این سه آفت است.


عنوان مقاله [English]

Optimization of using pesticide chlorpyrifos against Plodia interpunctella, Anagasta kuehniella and Galleria mellonella by response surface method under laboratory conditions

نویسندگان [English]

  • A. Shabani nejad
  • M. Ajam hassani
Faculty of Agriculture, Shahrood University of Technology, Shahrood
چکیده [English]

The Indian meal moth, Plodia interpunctella H., Mediterranean flour moth, Anagasta kuehniella Z., and Greater wax moth, Galleria mellonella L. are the most important pests of stored products and beehives. Chemical pesticides are commonly used to control them. Inappropriate using of pesticides caused serious environmental problems and also chronic effects on human health. Pest damage can be reduced by the optimum use of these materials. The purpose of optimum use is selection of the correct pattern and applying appropriate methods for use of pesticides. In this study Response Surface Method was used in order to determine optimal points to achieve maximum mortality with minimum concentration.  The effect of factors such as temperature (25-35°C), humidity (60-80 %) and the concentration (1400-2000 µL) was evaluated on the mortality of fifth instar larvae of three pests. The experiments were performed according to Central Composite Design. Temperature and dosage had significant effect on mortality of fifth instar larvae of three Pest. The Optimum conditions in minimum dosage to obtain the maximum mortalities were determined 7.9 fifth instar larvae of Indian meal moth are: 35 ° C, humidity 80% and 1400 microliter of chlorpyrifos in the 1000 ml of water and for Mediterranean flour moth 7.1 fifth instar larvae are: 35 ° C, humidity 75% and 1400 microliter in the 1000 ml of water and for Greater Wax Moth are7.9 fifth instar larvae are: 35 ° C, humidity 80% and 1500 microliter in the 1000 ml of water. Results show leaner and significant effect of concentrations and term on lethal fifth instar larvae of three pests.

کلیدواژه‌ها [English]

  • Stored pests
  • Response Surface Method
  • Chlorpyrifos
  • Optimization
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