بررسی میزان سازگاری برخی از اسانس های گیاهی با قارچ بیمارگر حشرات Beauveria bassiana علیه سوسک چهارنقطه ای حبوبات (Callosobruchus maculatus (Col.: Bruchidae

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه شهید مدنی آذربایجان، تبریز

2 گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه شهید مدنی آذربایجان، تبریز

3 گروه شیمی، دانشکده علوم پایه، دانشگاه شهید مدنی آذربایجان، تبریز

چکیده

به­ منظور ارزیابی میزان سازگاری چند اسانس گیاهی با قارچ بیمارگر حشرات Beauveria bassiana در کاربرد توأم علیه سوسک چهارنقطه ­ای حبوبات، این پژوهش طراحی و اجرا شد. تاثیر کشندگی سه اسانس شامل جعفری Petroselinum sativum، مرزه‌ سهندی Satureja sahendica  و گلپر ایرانی Heracleum persicum  روی حشرات کامل Callosobruchus maculatus ارزیابی شد. هم­چنین میزان فعالیت زیستی دو جدایه IS-1 و IS-75 از قارچ B. bassiana در کنترل آفت به­ صورت جدا و نیز در درتلفیق با اسانس‌های فوق مورد مطالعه قرار گرفت. نتایج نشان داد در بین اسانس ­های گیاهی، اسانس مرزه‌ سهندی سمی‌ترین ترکیب روی حشرات کامل بوده و جدایه IS-75 قارچ در کاربرد به­ صورت تنها، زهرآگینی بیش­تری نسبت به جدایه IS-1 دارد. نتایج مربوط به درصد بازدارندگی از اسپورزایی و رشد میسلیومی قارچ نشان داد اسانس جعفری با 100 درصد بازدارندگی به­ طور کامل مانع از رشد رویشی و اسپورزایی این قارچ شد و اسانس مرزه‌ سهندی با 24 درصد بازدارندگی سازگاری بیش­تری نسبت به دو اسانس دیگر با قارچ عامل بیمارگر داشت. کاربرد توأم هر سه اسانس گیاهی و قارچ IS-75 در غلظت­ های کشنده و زیرکشنده نشان داد که اسانس گلپر ایرانی کم­ترین مقدار LT50 در اختلاط با این قارچ را دارد. برهمکنش اسانس- بیمارگر در اختلاط غلظت­ های زیرکشنده هر یک از اسانس­ های گیاهی با جدایه IS-75 قارچ نشان داد که فقط اختلاط مقادیر LC25 هر یک از عوامل دارای اثر هم­ افزایی در افزایش درصد مرگ و میر در جمعیت حشرات کامل بوده و سایر اختلاط­ های زیرکشنده، دارای اثرات تجمعی و یا آنتاگونیستی بودند.

کلیدواژه‌ها


عنوان مقاله [English]

Compatibility of some plant essential oils in combination with the entomopathogenic fungus, Beauveria bassiana against Callosobruchus maculatus (Col.: Bruchidae)

نویسندگان [English]

  • R. Hosseinzadeh 1
  • A. Mehrvar 2
  • N. Eivazian Kary 1
  • H. Valizadeh 3
1 Department of Plant Protection, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz
2 Department of Plant Protection, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz
3 Department of Chemistry, Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz
چکیده [English]

This study was conducted to evaluate the compatibility of some plant essential oils in combination with entomopathogenic fungus, Beauveria bassiana against Callosobruchus maculatus. Lethal effects of three essential oils including Petroselinum sativum, Satureja sahendica and Heracleum persicum against the adults of C. maculatus were studied. The biological activity of two fungal isolates, IS-1 and IS-75, as alone and in combination with the essential oils for insect pest control was evaluated. Results showed that S. sahendica essential oil was the most toxic agent on adults than other oils, and IS-75 isolate showed more virulence against the pest compared to IS-1 when applied alone. Results of percent inhibition of sporulation and mycelial growth of the fungus showed P. sativum essential oil with 100 percent inhibition completely inhibited fungal growth and sporulation and S. sahendica essential oil with 24 percent inhibition had more compatibility compared to two other essential oils. Combined application of three essential oils and IS-75 isolate of the fungus in lethal and sublethal concentrations showed that H. persicum essential oil has the least LT50 value in combination with the fungus.Essential oil-pathogen interactions of sublethal concentrations of plant essential oils and IS-75 fungal isolate revealed that the synergistic effects on percent adults’ mortality only belongs to the combination of LC25 concentrations of each of the agents, and other sublethal combinations had additive and antagonistic effects.  

کلیدواژه‌ها [English]

  • Plant essential oils
  • Entomopathogenic fungus
  • Synergistic ratio
  • Callosobruchus maculatus
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