کارایی باکتری Bacillus thuringiensis در کنترل بیولوژیک کرم میوه‌خوار خرما Batrachedra amydraula در شرایط مزرعه‌ای

نویسنده

سازمان تحقیقات، آموزش و ترویج کشاورزی، مؤسسه تحقیقات علوم باغبانی، پژوهشکده خرما و میوه های گرمسیری

چکیده

شب ­پره کوچک خرما (Batrachedraamydraula Meyrick) یکی از مهم­ترین آفات نخلستان می­ باشد. هدف از انجام این پژوهش بررسی کارایی باکتری Bacillus thuringiensis برای کنترل آن در شرایط نخلستان بود. برای انجام این آزمایش از جدایه kurstaki B. thuringiensis استفاده شد. از طرح اسپلیت پلات برای انجام تحقیق استفاده شد. پلات‌های اصلی شامل سه زمان انجام تیمار شامل همزمان، سه روز و یک هفته پس از ظهور آفت در نخلستان بود. پلات‌های فرعی شامل سه سطح مختلف غلظت حداقل، متوسط و حداکثر به ­ترتیب با غلظت یک دهم معادل و ده برابر غلظت کشنده 50 درصد که به صورت 2 روز در میان و به مدت یک هفته ادامه داشت. این آزمایش دارای چهار تکرار شامل یک نفر نخل رقم سایر بود. یک تیمار کنترل شیمیایی و یک تیمار بدون کنترل به عنوان شاهد در نظر گرفته شد. درصد آسیب میوه از اواسط فروردین ماه به فاصله هر دو هفته یک بار نمونه­ برداری شد. نتایج نشان داد که بین تیمارهای مختلف باکتری Bt، از نظر شدت آلودگی میوه اختلاف وجود دارد. به طوری که بیش­ترین آلودگی در تیمار غلظت حداقل در شرایط 7 و 3 روز پس از ظهور کرم میوه خوار ثبت شده است. کم­ترین آلودگی در شرایط غلظت حداکثر بود و از این لحاظ تفاوت معنی­ داری بین سه زمان مختلف انجام تیمار وجود ندارد. کم­ترین متوسط سرعت رشد و بیش­ترین کاهش متوسط سرعت رشد آسیب در تیمار غلظت حداکثر و همزمان با ظهور آفت بود. بالاترین کارایی کنترل به ­ترتیب در شرایط غلظت حداکثر هم­زمان با ظهور آفت بوده است.

کلیدواژه‌ها


عنوان مقاله [English]

Efficiency of Bacillus thuringiensis for biological control of date lesser moth (Batrachedra amydraula) in field conditions

نویسنده [English]

  • M. Latifian
Agriculture Research, Education and Extension Organization, Horticultural Science Research Institute, Date palm and tropical fruits research Center
چکیده [English]

The lesser moth, Batrachedra amydraula is one of the most important pests of Dates. The aim of this study was to investigate the efficiency of Bacillus thuringiensis to control this pest in Date palm plantation condition. Krustaki bacterial isolate was used. The split plot design was used to conduct the research. Main plots consisted of three times of treatments including same time, three days and a week after the appearance of the pest. Sub plots were three different concentrations medium, average and maximum including One-tenth, equal and ten times of LD50 given 2 days and continued for a week. The experiment consisted of four replicates on a Sayer Date palm tree. A chemical control (Diazinon insecticide) and a treated without the chemical and biological control was considered as control group. Percentage of damaged fruit as the symptoms of biological pest activity were been sampling from mid-April once every two weeks of treatment. The results showed that there were significant differences between treatments, so that the maximum infection was recorded on minimum concentration at 3 and 7 days after the lesser moth appearance. The minimum infestation was recorded on maximum concentration and there were no significant differences between the three different times of release. The lowest average growth rate and the highest average reduction of injury growth rate were recorded on maximum concentration and coincided with the pest emergence treatment. The highest performance control was recorded on the maximum concentration coincided with the emergence.

کلیدواژه‌ها [English]

  • The leeser Date moth
  • Bacillus thuringiensis
  • Efficiency of Control
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