بررسی ساختار ژنتیکی شته سبز مرکبات، (Aphis spiraecola Patch (Hem.: Aphididae، روی درختان مرکبات در شمال ایران

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران

2 پژوهشکده مرکبات و میوه‌های نیمه‌گرمسیری، مؤسسه تحقیقات باغبانی، سازمان تحقیقات،آموزش و ترویج کشاورزی، رامسر، ایران

3 گروه زراعت و اصلاح نباتات، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

چکیده

شته سبز مرکبات، Aphis spiraecola Patch (Hemiptera: Aphididae)، یکی از آفات مهم مرکبات در شمال کشور است. این آفت به طور مستقیم با تغذیه از شیره گیاهی و ترشح عسلک وبه طور غیر مستقیم با انتقال چندین ویروس گیاهی باعث خسارت به مرکبات می‌شود. هدف از این مطالعه تعیین ارتباط ساختار ژنتیکی جمعیت‌های مختلف شته  A. spiraecolaبا پراکنش جغرافیایی آن روی درختان مرکبات در شمال ایران بود. بدین منظور تنوع ژنتیکی شته‌های جمع‌آوری شده از هشت محل در شمال ایران با استفاده از هفت نشانگر ریزماهواره مورد بررسی قرار گرفت. در میان 271 فرد شته 195 ژنوتیپ منحصر به فرد شناسایی شد و میزان تنوع کلونال در جمعیت‌ها بین 93/0 - 5/0 بود که نشان­دهنده تولید مثل پارتنوژنز اختیاری در جمعیت‌های این شته در شمال ایران است. میانگین محتوای اطلاعات چند شکلی (PIC) 62/0 بود. انحراف از تعادل هاردی - وینبرگ در همه جمعیت‌ها وجود داشت. تجزیه واریانس مولکولی نشان داد تنوع ژنتیکی قابل توجهی داخل جمعیت‌ها و درصد ناچیزی از تنوع ژنتیکی به بین جمعیت‌های مربوط به مکان‌های مختلف جغرافیایی اختصاص دارد. همچنین بر اساس ماتریس فاصله ژنتیکی نی، در بین جمعیت‌ها فاصله ژنتیکی قابل توجهی مشاهد نشد که با نتایج تجزیه ساختار با استفاده از نرم افزارStructure  نیز مطابقت داشت. یافته‌های این پژوهش اطلاعات کاربردی درباره پراکنش ژنوتیپی شته سبز مرکبات در شمال کشور فراهم می‌کند که در ترکیب با اطلاعات موجود از چرخه زندگی آن می‌تواند در برنامه‌های مدیریت کنترل آفت مورد استفاده قرار گیرد.

کلیدواژه‌ها


عنوان مقاله [English]

Population genetic structure of Aphis spiraecola Patch (Hem.: Aphididae) on citrus trees in Northern Iran

نویسندگان [English]

  • E. Gholamian 1
  • J. Razmjou 1
  • M. Banihashemian 2
  • A. Sabouri 3
1 Department of Plant Protection, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran
2 Citrus and Subtropical Fruits Research Center, Horticultural Science Research Institute, Agricultural Research, Education and Extension Organization, Ramsar, Iran
3 Department of Agronomy & Plant Breeding, Faculty of Agriculture, University of Guilan, Rasht, Iran
چکیده [English]

The spirea aphid, Aphis spiraecola Patch (Hemiptera: Aphididae), is a serious pest of citrus in northern Iran. It causes direct damage by sap feeding and extracting honeydew and also indirect damage by transmission of several virus diseases. The aim of this study was determining the relationship between genetic structure of different populations of A.spiraecola with its geographical distribution on citrus trees in northern Iran. The genetic structure of A. spiraecola collected on citrus trees in eight localities, was described by seven polymorphic microsatellite loci. Among 271 individuals, 195 multilocus genotypes were identified and the clonal diversity in populations were 0.5 to 0.93. They confirmed that A. spiraecola reproduces by cyclical parthenogenesis on citrus trees in North of Iran. The mean of Polymorphic Information Content was (PIC =0.62). There were significant deviations from Hardy-Weinberg equilibrium in all populations. The analysis of molecular variance revealed high intrapopulation and weak interpopulation genetic differentiation among geographic populations. Also, the UPGMA dendrogram of 8 populations based on Nei's genetic distance indicated no considerable genetic differentiation among them, which is in accordance with structural analysis. The findings of this research provide useful information about the genotypic distribution of A. spiraecola in Northern Iran which can be integrated with life cycle information of the pest and used in pest management programs.

کلیدواژه‌ها [English]

  • Microsatellite
  • genetic differentiation
  • Aphid
  • clonal diversity
  • cyclical parthenogenesis
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