Effect of Bacillus thuringiensis, SeNPV, Spinosad and Emamectin on third larval instar of Spodoptera exigua (Lep.: Noctuidae) in laboratory and field conditions

Document Type : Research Paper

Authors

1 Department of Plant Protection, Agricultural Faculty, Urmia University, Iran

2 Department of Plant Protection, Mahabad Branch, Islamic Azad University, Mahabad, Iran

Abstract

The beet armyworm, Spodoptera exigua Hübner is one of the most important pests of sugar beet. Due to the problems associated with the use of chemical pesticides against of this pest, the use of bio-pesticides in integrated management program is recommended. Therefore, in this research, effects of Bacillus thuringiensis subsp. kurstaki, SeNPV virus, Spinosad and Emamectin on 3rd larval instar of beet armyworm in laboratory and field conditions were evaluated. In laboratory experiments, LC50 values of different concentrations of B. thuringiensis, SeNPV virus, Spinosad and Emamectin after 24, 48 and 72 hours were determined by probit analysis. Also, the effects of each compound using Henderson- Tilton method and GLM analysis after 1, 3, 7, 14 and 21 days were evaluated in field conditions. The results of probit analysis of different concentrations of Bt., Spinosad and Emamectin after 24, 48 and 72 hours were (3588.5, 1.25, 1.89), (1954.87, 0.95, 1.33) and (1843.25, 0.78, 1.20) mg/L, and for SeNPV (5.34×105, 4.31×105, 4.14 ×105) OBs.ml, respectively. Also, the highest and lowest mortality due to Bt., SeNPV, Spinosad and Emamectin in field conditions after 21 days were related to Emamectin (61.33) and SeNPV virus (32.00) %. The highest and lowest percentages of damage were observed in the SeNPV virus, control and the Emamectin and Spinosad, respectively. Based on laboratory and field bioassay, Spinosad and Emamectin, as the most effective compounds for controlling this pest and virus and Bt for integrated pest management are recommended.

Keywords


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