Effect of a lectin extracted from Polygonum persicaria L. on some compounds involved in intermediary metabolism of Pieris brassicae L. (Lep.: Pieridae) larvae

Document Type : Research Paper

Authors

1 Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Tea Research Center, Horticulture Science Research Institute; Agricultural Research, Education and Extension Organization (AREEO), Lahijan, Iran

3 Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

Abstract

            Lectins are the proteins with the heterogenous structure that bind to mono- or oligosaccharides of cell surfaces leading to interference in cellular processes or even death. In the current study, effects of the different concentrations (0.5, 1 and 2 mg/ml) of Polygonum persicaria L. lectin were assessed on the intermediary metabolism of Pieris brassicae L. larvae to better understanding of its entomotoxic mechanism. Feeding of the larvae on the Polygonum persicaria lectin increased activities of aspartate aminotransferase and gamma-glutamyl transferase but the activity of alanine aminotransferase statistically decreased. Activity of aldolase increased in the treated larvae in a dose-dependent manner. Also, activity of acid phosphatase showed no statistical differences between treated and control larvae, but alkaline phosphatase in the larvae fed on 2 mg/ml of lectin had the highest activity in comparison with other treatments. High density lipoprotein in the larvae fed on 2 mg/ml of lectin, had the lowest amount but low density lipoprotein showed the highest amount. Among storage macromolecules, glycogen showed no statistical difference between control and treated larvae but the amounts of protein and triglyceride statistically decreased. Results of the current study showed that P. persicaria lectin can cause disturbance in intermediary metabolism of P. brassicae larvae in its highest use dose which may lead lower biological and reproductive efficiencies.

Keywords

References

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Plant Pest Research
Volume 6, Issue 4
March 2017
Pages 17-29

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  • Receive Date: 13 March 2017
  • Accept Date: 13 March 2017

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