کارایی فرمولاسیون‌های EC چریش (28/1%) و نیمارین® (1%) روی لیسک Agriolimax agrestis در شرایط آزمایشگاهی و گلخانه‌ای

نوع مقاله : مقاله پژوهشی


1 بخش تحقیقات جانورشناسی کشاورزی، موسسه تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران

2 بخش تحقیقات گیاه‌پزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان مازندران، سازمان تحقیقات، آموزش و ترویج کشاورزی، ساری، ایران

3 بخش تحقیقات گیاه‌پزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان گیلان، سازمان تحقیقات، آموزش و ترویج کشاورزی، رشت، ایران

4 بخش تحقیقات آفت‌کش‌ها، موسسه تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران


تاثیر فرمولاسیون­های جدید چریش، نیمارین® و متالدئید بر لیسک  A. agrestisدر شرایط آزمایشگاهی و گلخانه­ای ارزیابی شد. در آزمایش­های زیست­سنجی، برگ­های گیاه زینتی اسپاتیفیلوم (Spathiphyllum wallisii Regel)در غلظت­های مختلف چریش و نیمارین® غوطه‌ور و سپس لیسک­های هم‌سن نابالغ روی برگ­ها رهاسازی شده و میزان مرگ و میر و تغذیه آن­ها تا 14 روز تعیین شد. مقادیر LC50 برای چریش و نیمارین® به ترتیب 1/34 و 5/22 در هزار و سمیت نسبی نیمارین®59/1 برابر چریش برآورد شد. آزمایش‌های گلخانه­ای در قالب طرح کاملاً تصادفی در 4 تیمار انجام و بیش­ترین تاثیر تیمارها بعد از 15 روز مربوط به متالدئید با میانگین مرگ و میر 75/95 درصد در مقایسه با چریش و نیمارین®به ترتیب با میانگین‌های 85/47 و 63/51 درصد به­دست آمد. همچنین، تاثیر چریش و نیمارین® بر تغذیه به ترتیب 72/1 و 9/1 گرم در مقایسه با شاهد (9 گرم) بود و درصد تفریخ تخم در مقایسه با شاهد به­صورت معنی­داری کاهش یافت. بر اساس نتایج، ترکیبات چریش و نیمارین® به ترتیب در غلظت‌های 1/34 و 5/22 در هزار و تا ده برابر آن روی برگ‌های اسپاتیفیلوم، در تغذیه A. agrestis اثر منفی داشته و سبب کاهش خسارت آن در شرایط گلخانه­ای شد. این اثر با افزایش سن و جثه لیسک و در پی آن افزایش غلظت، حفظ شد. با این حال ترکیبات مذکور از لحاظ کشندگی در مقایسه با سم متالدئید ضعیف­تر عمل کردند.


عنوان مقاله [English]

Efficacy of the EC formulations of Neem (1.28%) and Neemarin® (1%) on slug Agriolimax agrestis in laboratory and greenhouse conditions

نویسندگان [English]

  • E. Ahmadi 1
  • M. GholamzadehChitgar 2
  • Z. Mojib Hagh Ghadam 3
  • A. Heidari 4
1 Agricultural Zoology Research Department, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
2 Plant Protection Research Department, Mazandaran Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization, Sari, Iran
3 Plant Protection Research Department, Gilan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization, Rasht, Iran
4 Pesticides Research Department, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.
چکیده [English]

The effect of new formulations of Neem, Neemarin® and metaldehyde on slug, A. agrestis was evaluated in laboratory and greenhouse conditions. In bioassay experiments, the leaves of the ornamental plant (Spathiphyllum wallisii Regel) were dipped in different concentrations of neem and Neemarin®, and then same-age immature slugs were released on the leaves and their mortality and feeding were determined up to 14 days. The LC50 values for Neem and Neemarin® were estimated 34.1 and 22.5 ml/lit, respectively, and relative toxicity of Neemarin® was 1.59 times of /more than Neem. The greenhouse experiments were conducted in RCD with 4 treatments, and the maximum effect of the treatments was obtained for metaldehyde with a mean of 95.75% after 15 days compared to the Neem and Neemarin® with 47.85% and 51.63% mean mortality, respectively. Also, the effect of Neem and Neemarin® on the nutrition was 1.72 g and 1.9 g, respectively, in comparison with control (9 g) and the percentage of egg hatching significantly decreased compared to the control. According to the results, Neem and Neemarin® compounds in the concentrations 34.1 and 22.5 per thousand, respectively, and up to ten times on leaves of S. wallisii had a negative effect on the nutrition of A. agrestis and reduced the damage in greenhouse conditions. This effect with increasing age and body size of the slug and subsequent increase in concentration was maintained. However, they were less lethal than methaldehyde.

کلیدواژه‌ها [English]

  • Agriolimax agrestis
  • Neem
  • Neemarin®
  • Metaldehyde
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