Effect of casein and zein as additives on some nutritional and immunological indices of Ephestia kuehniella Zeller (Lep: Pyralidae)

Authors

Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan

Abstract

The eggs and larvae of the Mediterranean flour moth, Ephestia kuehniella (Zell.), are widely used to rear parasitoids and predators for biological control programs. In this study, nutritional responses and digestive enzymes activities of the fourth instar larvae, as well as some immunological parameters of E. kuehniella, were studied on the diet containing casein and zein proteins under laboratory conditions. The fourth larval instar reared on diet containing casein had the highest relative rate of consumption (5.9 ± 0.2) and relative growth rate (11.3 ± 0.3). The highest and the lowest activity of lipase, tripcine, elastase, carboxypeptidase and aminopeptidase in larvae fed were on casein and zein proteins. In the immunological study, injection of tween and Beauveria bassiana (Balsamo) Vuillemin in larvae treated by casein protein significantly increased the total number of hemocyte and the number of granulocytes and plasmatocytes. This study indicated that the nature of the protein in the diet effects on physiological mechanisms of feeding and affect the immune response. 

Keywords

References

Abdi, A., Naseri, B. and Fathi, S. 2014. Nutritional indices, and proteolytic and digestive amylolytic activities of Ephestia kuehniella (Lep.: Pyralidae): response to flour of nine wheat cultivars. Journal of Entomological Society of Iran 33(4): 29-41.
Abisgold, J. D. and Simpson, S. J. 1987. The physiology of compensation by locusts for changes in dietary protein. Journal of Experimental Biology 129: 329-346.
Abroun, P., Mousavi, S. Gh., Ashouri, A. and Gishani, H. 2013. Effect of different quality of Ephestia kuehniella on the parasitism of Trichogramma brassicae. The 1st National Conference on Sustainable Agriculture and Natural Resources. 1-8. (in Farsi)
Baker, J. E. and Woo, S. M. 1985. Purification and partial characterization and postembryonic levels of amylase from Sitophilus orizae and Sitophilus granarius. Archives of Insect Biochemistry and Physiology 2: 415-428.
Bandani, A. 2013. Insect Physiology (Digestion, Excretion, Symbiont Microorganisms and Metabolism). 2nd Edition. University of Tehran Press. 459.
Batista Pereira, G. L., Petacci, F., Fernandes, B. J., Correa, A. G., Vieira, P. C., Fatima da Silva, M. and Malaspina, O. 2002. Biological activity of astilbin from Dimorphandra mollis against Anticarsia gemmatalis and Spodoptera frugiperda. Pest Management Science58: 503-507.
Beckage, N. E. 2008 Insect Immunology, Academic Press. California.
Bidar, F. 2014. Nutritional reaction and digestive enzymes activity of Ephestia kuhnila Zeller (Lep: Pyralidae) on flour of different cultivars. Msc., thesis. The University of Mohagegh Ardebili. (In Farsi)
Bulet, P. and Stocklin, R. 2005. Insect antimicrobial peptides: structures, properties and gene regulation. Protein and Peptide Letters 12(1): 3-11.
Chapman, R. F. 1998. The Insects Structure and function, 4th edition, Cambridge University Press, Cambridge. 782.
Correia, A. A. 2008. Histofisiologia do canal alimentar e hemócitos de Spodoptera frugiperda (JE Smith) (Lepdoptera: Noctuidae) tratadas com nim (Azadirachta indica A. Juss). Dissertation, Universidade Federal Rural de Pernambuco.
Felton, G. W. 1996. Nutritive quality of plant protein: sources of variation and insect herbivore responses.Insect Biochemistry Physiology32: 107-130.
Haine, E. R., Pollitt, L. C., Moret, Y., Siva-Jothy, M. T. and Rolff, J .2008. Temporal patterns in immune responses to a range of microbial insults (Tenebrio molitor). Journal of Insect Physiology 54(6): 1090-1097.
Hilder, V. A., Gatehouse, A. M. R. and Boulter, D. 1992. Transgenic plants conferring insect tolerance: proteinase inhibitor approach. In: Transgenic Plants, Vol 1. Academic Press, New York, pp: 317-338.
Jacob, T. A. and Cox, P. D. 1977. The influence of temperature and humidity on the life-cycle of Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Journal of Stored Products Research 13: 107-118.
Jafarlu, M., Farshbaf Pourabad, R. and Valizadeh, M. 2013. Effect of Different Compounds on Midgut α-Amylase Activity of the Mediterranean FlourMoth, Anagasta kuehniella (Zeller, 1879) (Lep., Pyralidae). Applied research in plant protection 2: 1. (In Farsi).
Johnson, J. A., Valero, K. A. and Hannel, M. M. 1997. Effect of low temperature storage on survival and reproduction of Indian meal moth (Lepidoptera: Pyralidae). Journal of Crop Protection 16: 519-523.
Jouanin, L., Bonadé-Bottino, M., Girard, C., Morrot, G. and Giband, M. 1998. Transgenic plants for insect resistance. Plant Science 131(1): 1-11.
Koul, O., Singh, G., Sing, R. and Singh, J. 2004. Bioefficacy and mode-of-action of some limonoids of salanin group from Azadirachta indica, A. Juss and their role in a multicomponent system against lepidopteran larvae. Journal of Bioscience 29: 409-416.
Lavine, M. and M. Strand. 2002. Insect hemocytes and their role in immunity. Insect Biochemistry and Molecular Biology 32: 1295-1309.
Lazarevic, J. and Peric-Mataruga, V. 2003. Nutritive stress effects on growth and digestive physiology of Lymantria dispar larvae. Yugoslav Medical Biochemistry22: 53-59.
Lee, K. P. 2007. The interactive effects of protein quality and macronutrient imbalance on nutrient balancing in an insect herbivore. Journal of Experimental Biology 210: 3236-3244.
Lee, K. P., Simpson, S. J. and Wilson, K. 2008. Dietary protein -quality influences melanization and immune function in an insect. Functional Ecology 22: 1052-1061.
Mayrand, E., St-Jena, S. D. and Courtenay, S. C. 2005. Haemocyte responses of blue mussels (Mytilus edulis L.) transferred from a contaminated site to a reference site: can the immune system recuperate? Aquaculture Research 36: 962-971.
Moradi-Naserabadi, N. 2018.Comparison of Phenol Oxidase enzyme activity in hemocyte cell of Ephestia kuehniella Zeller (Lep.: Pyralidae) in three different diets and the effect of Bacillus thuringiensis Endotoxin on it. MSc. Thesis. The University of Tabriz. (In Farsi)
Mullin, M. M. and Brooks, J. E. R. 1970. Growth and metabolism of two planktonic, marine copepods as influenced by temperature and type of food. In Steele J. H. (ed.), Marine Food Chains. University of California Press, Berkeley, CA, pp. 74-95.
Nasirian, R., Naseri, B. and Razmjou, J. 2014. Feeding performance and some biological parameters of the Indian meal moth, Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae) on artificial diets containing bran of different wheat cultivars. Journal of Crop Protection 3(3): 295-304.
Nathan, S. S., Chung, P. G. and Murugan, K. 2005. Effect of biopesticides applied separately or together on nutritional indices of the rice leaf older Cnaphalocrocis medinalis. Phytoparasitica 33: 187-195.
Nouri-Ganbalani, G., Borzoui, E., Nouri, A. and Tajmiri, P. 2017.Effect of different potato cultivars on nutritional indices and activity of some digestive enzymes of Leptinotarsa decemlineata (Col.: Chrysomelidae). Iranian Journal of Plant Protection Science 48(1): 109-118
Oppert, B., Kramer, K. J. and McGaughey, W. H. 1997. Rapid microplate assay of proteinase mixtures. Journal of Biotechnology 23: 70-72.
Panizzi, A. R. and Parra, J. R. P.1991. Ecologia nutricional de insetos esua aplicacao no manejo de Pragas. Manole/ CNPq, Sao Paula, 359 p.
Pech, L. L. and Strand, M. R .1996. Granular cells are required for encapsulation of foreign targets by insect haemocytes. Journal of Cell Science 109(8): 2053-2060.
Rezaei, A. M. and Soltani, A. 2001. Potato Crop Cultivation. SID Publisher. (3th ed). 179 p.
Singh, O. P. and Parihar, S. B. B. 1988. Effect of different hosts on the development of Heliothis armigera Hub. Bulletin of Entomology 29: 168-172.
Srinivasan, R. and Uthamasamy, S. 2005. Trichome density and antibiosis affect resistance of tomato to fruitborer and whitefly under laboratory conditions. Journal of vegetable science 11(2): 3-17.
Tsujita, T., Ninomiya, H. and Okuda, H. 1989. P-nitrophenyl butyrate hydrolyzing activity of hormone-sensitive lipase from bovine adipose tissue. Journal of Lipid Research 30: 997–1004.
Vanderzant, E. S. 1966 .Development, significance and application of artificial diet for insect. Annual Review of Entomology 19: 139-405.
Vijverberg, J.1989. Culture techniques for studies on the growth, development and reproduction of copepods and cladocerans under laboratory and in situ conditions. a review. Freshwater Biology21, 317-373.
Waldbauer, G. P. 1968 The consumption and utilization of food by insects. Advances in Insect Physiology5: 229-288.
Plant Pest Research
Volume 8, Issue 2
September 2018
Pages 25-39

Files

History

  • Receive Date: 15 September 2018
  • Accept Date: 15 September 2018

Share

How to cite

Statistics