کارایی زنبور پارازیتوئیدHabrobracon hebetor در تلفیق با ماترین و باکتری Bacillus thuringiensis در کنترل Anagasta kuehniella

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه ارومیه، ایران

2 گروه گیاه‌پزشکی، دانشکده کشاورزی، واحد مهاباد، دانشگاه آزاد اسلامی، مهاباد، ایران

3 گروه حشره شناسی، مرکز تحقیقات کشاورزی هیس، دانشگاه کانزاس. امریکا

چکیده

 در این تحقیق، کارایی زنبور پارازیتوئید­ Habrobracon hebetor (Say) در تلفیق با ماترین و باکتریBacillus thuringiensis (Berliner) subsp. Kurstaki (Btk) در کنترلAnagasta kuehniella (Zeller) ، بررسی شد. غلظت­های کشنده و زیر­کشنده (LC30) ماترین و Btk  با روش زیست­سنجی روی لارو میزبان تعیین شد. برای ارزیابی ترجیح سن میزبانی توسط پارازیتوئید، با در اختیار قرار دادن لاروهای سنین مختلف میزبان، میزان پارازیتیسم زنبور تعیین شد. لاروهای سن چهارم و پنجم میزبان با غلظت زیر­کشنده ماترین و Btk تیمار شدند و در حالت­های انتخابی و غیرانتخابی، در اختیار زنبور پارازیتوئید ­قرار گرفتند. سپس میزان فلج کنندگی، پارازیتیسم­، تخم­گذاری، درصد ظهور و نسبت جنسی زنبورهای خارج شده تعیین شد. نتایج نشان دادکه زنبور­های ماده، لاروهای سنین چهارم و پنجم میزبان را برای پارازیتیسم ترجیح می دهند.  در آزمون انتخابی زنبور­های ماده، لاروهای تیمار شده با ماترین را برای پارازیتیسم ترجیح دادند اما در آزمون غیرانتخابی، بیشترین میزان پارازیتیسم و تخم‌گذاری مربوط به تیمار شاهد بود. درصد ظهور زنبورهای بالغ و نسبت جنسی آن­ها (ماده / کل)، حاصل از لاروهای سن چهارم و پنجم تیمار شده با ماترین به ­ترتیب در آزمون انتخابی 61 و 54 درصد و آزمون غیرانتخابی 64 و 54 درصد بودند. نتایج نشان داد ماترین و Btk پتانسیل قابل توجهی در کنترل A. kuehniella دارند. اما با توجه به اثرات سوء زیر­کشنده این عوامل کنترلی بر کارایی پارازیتوئید، کاربرد هم­زمان آن­ها با زنبور پارازیتوئید H. hebetor، پیشنهاد نمی­ شود.

کلیدواژه‌ها

عنوان مقاله [English]

Efficacy of the parasitoid wasp, Habrobracon hebetor in integrating with Matrine and Bacillus thuringiensis in the control of Anagasta kuehniella

نویسندگان [English]

  • M. Azimi 1
  • Sh. Aramideh 1
  • Sh. Mirfakhraie 1
  • A. Hosseinzadeh 2
  • J. P., Michaud 3
1 Department of Plant Protection, Urmia University, Urmia, Iran,
2 Department of Plant Protection, Mahabad Branch, Islamic Azad University, Mahabad, Iran
3 Department of Entomology, Kansas State University, Agricultural Research Center-Hays. USA
چکیده [English]

In this research, the efficiency of the parasitoid wasp, Habrobracon hebetor (Say) in integrated with Matrine and Bacillus thuringiensis (Berliner) subsp. Kurstaki (Btk) against Anagasta kuehniella (Zeller), was investigated. Lethal and sublethal concentrations (LC30) of Matrine and Btk were determined by bioassay method on host larvae. To evaluate the preference of the host larval instars by the parasitoid, different larval instars were provided for parasitoid and the rate of parasitism was determined. The fourth and the fifth instar larvae of the host were treated with sublethal concentrations of Matrine and Btk then were provided for parasitoid in choice and non- choice conditions. The rate of paralysis, parasitism, egg-laying, emergence percentage and sex ratio of release wasps were determined. The results showed that female parasitoid preferred the fourth and fifth instar larvae of the host for parasitism. In the choice test, Matrine-treated larvae were more preferred by female parasitoid while in non-choice test, the highest amount of parasitism and egg-laying was related to the control treatment. The emergence percentage of adult wasps and their sex ratio (Female/Total), obtained from Matrine-treated fourth and fifth instar larvae, were 61 and 54% in the choice and 61 and 54% in the non-choice tests. The results showed that Matrine and Btk have significant potential in controlling of A. kuehniella. However, due to the sublethal adverse effects of these control agents on the efficiency of the parasitoid, simultaneous use of them with the parasitoid wasp, H. hebetor is not recommended.

کلیدواژه‌ها [English]

  • Biological control
  • host preference
  • integrated control
  • Mediterranean flour moth
  • sub-lethal effect

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تحقیقات آفات گیاهی
دوره 13، شماره 4
اسفند 1402
صفحه 1-15

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  • تاریخ دریافت: 03 آبان 1402
  • تاریخ بازنگری: 21 دی 1402
  • تاریخ پذیرش: 23 دی 1402

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