امکان استفاده از برخی جدایه‌های ایرانی قارچ بیمارگر Akanthomyces برای کنترل میکروبی شپشک آردآلود پنبه Phenacoccus solenopsis

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه گیاه‌پزشکی دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، شهرستان باوی، خوزستان، ایران

چکیده

شپشک آردآلود پنبه (Hemiptera: Pseudococcidae)Tinsely  Phenacoccus solenopsis  یکی از آفات مهم بسیاری از گیاهان در اکوسیستم­های کشاورزی و فضاهای سبز شهری می­باشد. در این مطالعه، امکان به کارگیری سه جدایه ایرانی از قارچ Akanthomyces lecanii (جدایه­های PAL6، PAL7 و PAL8) و یک جدایه ایرانی از گونه A. muscarius (جدایه GAM5) در کنترل میکروبی این آفت در شرایط گلخانه­ای و روی گیاه حسن یوسف (به عنوان میزبان گیاهی شپشک) مورد ارزیابی قرار گرفت. برای این منظور، گیاهان آلوده به پنج پوره­های سن آخر شپشک توسط غلظت­های مختلف (104، 105، 106، 107 و 108 کنیدی در هر میلی­لیتر) هر جدایه تیمار شده و میزان مرگ و میر شپشک­ها تا هشت روز و به صورت روزانه ثبت شد. میزان مرگ و میر از 3/14 درصد (در جدایه PAL7) تا 79/1 درصد (در جدایه PAL8) متغیر بود. بیشترین میزان مرگ و میر در غلظت 108 کنیدی در هر میلی­لیتر بیمارگرهای مزبور دیده شد. بیشترین مرگ و میر ناشی از بیماری­زایی جدایه­ها در روز ششم پس از تیمار ثبت شد. به طور کلی، سوسپانسیون کنیدی جدایه PAL7 قارچ A. lecanii با غلظت 108 کنیدی در هر میلی­لیتر بهترین گزینه بین بیمارگرهای مورد مطالعه برای کنترل میکروبی شپشک آردآلود پنبه بود که در شش روز پس از تیمار موجب 17/56 درصد مرگ و میر در جمعیت این آفت شد. از نتایج این تحقیق می­توان برای توسعه کنترل میکروبی شپشک آرد آلود پنبه به ویژه در محیط­های گلخانه­ای استفاده نمود.

کلیدواژه‌ها

عنوان مقاله [English]

Potential of some Irianian isolates of Akanthomyces for microbial control of cotton mealybug, Phenacoccus solenopsis, under greenhouse conditions

نویسندگان [English]

  • N. Lalari Sardar-Abadi
  • F. Yarahmadi
  • N. Zandi-Sohani
  • M. H. Ghodom Parizipour
  • A. Rajabpour
Department of Plant Protection, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Bavi, Iran
چکیده [English]

The cotton mealybug, Phenacoccus solenopsis Tinsely (Hemiptera: Pseudococcidae), is an important pest of many plants in agroecosystems and urban green landscapes. In this study, potential of three isolates of Akanthomyces lecanii, isolates PAL6, PAL7, and PAL8, and an isolate of A. muscarius, isolate GAM5, to microbial biocontrol of this pest on Coleus blumei, as the pest host plant, were investigated under greenhouse conditions. The infested host plants to five last instar nymphs were treated using different conidial suspension concentrations (104,105, 106, 107, and 108 conidia/mL) of each isolate and the mealybug mortalities were recorded every day, for eight days. The mortalities were varied from 14.3% (isolate PAL7) to 1.79% (isolate PAL8). The highest mortality was observed in concentration of 108 conidia/mL of the pathogens. The highest mortality caused by the pathogens was recorded six days after treatment. Totally, conidial suspension concentration of 108 conidia/mL from A. lecanii PAL7 was the best candidate for microbial biocontrol of the mealybug among the pathogens causing 56.17% mortality in the pest population six days after treatment. The results can be used for developing microbial biocontrol of the cotton mealybug especially in greenhouse conditions.

کلیدواژه‌ها [English]

  • Greenhouse pests
  • IPM
  • microbiological control
  • pathogenesis

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تحقیقات آفات گیاهی
دوره 12، شماره 3
آذر 1401
صفحه 57-70

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  • تاریخ دریافت: 19 آذر 1401
  • تاریخ پذیرش: 19 آذر 1401

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