افزایش فعالیت استرازها و گلوتاتیون-اس-ترانسفرازها در جمعیت‏ های مقاوم کنه قرمز مرکبات Panonychus citri (Acari: Tetranychidae) به آبامکتین

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان

2 دانشکده کشاورزی، دانشگاه صنعتی اصفهان

چکیده

کنه قرمز مرکبات Panonychus citri (McGregor) (Acari: Tetranychidae) یکی از آفات مهم مرکبات در مناطق مرکبات­خیز جهان و استان‏های شمالی ایران است. به کارگیری آفت‏کش‏های شیمیایی متعدد علیه آفات مرکبات موجب از بین رفتن دشمنان طبیعی، بروز پدیده‏ی مقاومت و طغیان مجدد این آفت شده است. در این تحقیق سازوکارهای مقاومت کنه قرمز مرکبات به آبامکتین در چهار جمعیت نمونه­برداری شده مورد بررسی قرار گرفت. برای تعیین میزان LC50 از روش برج پاشش استفاده شد که در این روش میزان مقاومت در جمعیت گرگان 48/6 برابر، رامسر 19/6 برابر و ساری 11/5 برابر بیشتر از جمعیت حساس تعیین شد. سنجش فعالیت استرازی با استفاده از سوبسترای آلفا-نفتیل استات و بتا-نفتیل استات در جمعیت مقاوم گرگان به ترتیب 960/7و 575/3 برابر بیشتر از جمعیت حساس بود. برآورد فراسنجه‏های سینتیکی و میزان فعالیت آنزیم گلوتاتیون-اس-ترانسفراز نیز تفاوت معنی‏داری بین چهار جمعیت نشان داد به طوری که میزان فعالیت این آنزیم در جمعیت مقاوم 79/2 برابر جمعیت حساس بود و مقادیر Km و Vmax با استفاده از سوبسترای 1-کلرو-2و4-دی‏نیتروبنزن (CDNB) به­ترتیب 21/16و 33/5 برابر بیشتر از جمعیت حساس تعیین شد. با توجه به روند افزایشی مقاومت، نتایج این تحقیق بیانگر نقش آنزیم‏های استرازی و گلوتاتیون-اس-ترانسفرازی در مقاومت به کنه‏کش آبامکتین هستند.

کلیدواژه‌ها

عنوان مقاله [English]

Enhanced activity of esterases and glutathion-s-transferases in abamectin resistant populations of Panonychus citri (Acari: Tetranychidae)

نویسندگان [English]

  • E. Shafiei Alavijeh 1
  • M. Ghadamyari 1
  • J. Khajeali 2
1 Department of Plant Protection, College of Agricultural Sciences, University of Guilan, Rasht, Iran
2 Department of Plant Protection, College of Agriculture, Isfahan University of Technology
چکیده [English]

Citrus red mite, Panonychus citri (McGregor) (Acari: Tetranychidae) is one of the most important pest of citrus in the citrus regions of the world and Northern provinces of Iran. Application of various chemical pesticides against citrus pests has destroyed natural enemies, the occurrence of resistance and the resurgence of the pests. In this research, resistance mechanisms of citrus red mite to abamectin were investigated in four populations. To determine the LC50, the spray potter tower method was used. In this method, the resistance level in Gorgan, Ramsar, and Sari populations was 6.48, 6.19 and 5.11 times higher than the susceptible population. The measurement of esterase activity in Gorgan's resistant population using α-naphthyl acetate and β-naphthyl acetate substrate was 7.960 and 3.575 times more than that of the susceptible population. Estimation of kinetic parameters and glutathione-s-transferase activity level using 1-chloro-2,4-dinitrobenzene (CDNB) as substrate also showed a significant difference between the four populations, so that the activity of this enzyme in resistance population was 2.79 fold higher than that of resistant population. Also, Km and Vmax in resistant population were 16.12 and 5.33 fold than susceptible population, respectively. Due to the increasing trend of resistance, the results of this study indicated the role of esterase and glutathione-s-transferase enzymes in resistance mechanism to abamectin.

کلیدواژه‌ها [English]

  • Citrus red mite
  • Esterase Activity
  • Glutathione S-transferas
  • Resistant

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تحقیقات آفات گیاهی
دوره 8، شماره 3
آذر 1397
صفحه 31-43

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  • تاریخ دریافت: 28 آذر 1397
  • تاریخ پذیرش: 28 آذر 1397

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