نوع مقاله : مقاله پژوهشی
نویسندگان
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Boll worm, Helicoverpa armigera (Hϋbner) (Lepidoptera: Noctuidae) is a cosmopolitan and polyphagous pest that attacks many plant species. The most important control method is based on the use of pesticides which is a concern to environment as well as insects' resistance, thus alternative ways to chemical ones are required. The aim of the current study is to investigate the effect of protein extract of triticale against boll worm amylase. To do so, fourth instar larvae were dissected, their gut removed and their amylase were extracted. Protein of triticale was extracted using 0.1 M NaCl. Effect of proteinaceous extracts on amylase activity in both enzymatic assay and in gel assay using zymogram was performed. Results showed that the inhibitory process was dose dependant. The highest (17 µg protein) and the lowest concentration (1.0625 µg protein) of triticale extracts produced 70 and 30% inhibition activity, respectively. The highest inhibitory activity was observed at pH 8. Dose dependant manner of inhibition also was observed in gel assay. At the highest concentration of the inhibitor, two bands were completely eliminated while the intensity of major band was reduced. When the lowest dose was used two bands was removed but intensity of major band was still high.
کلیدواژهها [English]
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